1～7日龄小鼠仔鼠海马神经元突触超微结构研究  

The researchment of hippocampus synapses ultra-structural of 1 to 7 days offspring of mice

作　　者：贾丽棉[1] 陈素华[2] 王玲[2] 张丹丹[2] 肖娟[2] 凌霞珍[2] 

机构地区：[1]河南省商丘市第一人民医院,476000 [2]华中科技大学同济医学院附属同济医院

出　　处：《中国医学创新》2010年第33期3-5,共3页Medical Innovation of China

摘　　要：目的观察1～7日龄小鼠仔鼠海马神经元突触结构超微变化。方法选择性成熟期BALB/C小鼠,雌雄同笼受孕,于自然分娩后1～7 d每日处死7只仔鼠,无菌剥离海马组织,电镜观察海马神经元突触超微结构变化。结果随日龄增加,海马神经元数目无明显变化,但海马神经元细胞器(线粒体、内质网等)数量逐渐增多、体积逐渐增大;突触界面曲率及突触后膜致密物厚度逐日迅速增加(P<0.05);突触间隙逐日增大,其中3～7日龄增大非常迅速(P<0.05)。结论海马神经元细胞器以及突触超微结构的变化,可能是出生早期仔鼠学习记忆能力快速发展的基础。Objective To observe the ultra - structural changes of mouse hippocampus synapses from birth to seven days. Methods The sexually mature mice were served as the subjects,and then put the males and females in the same cages. When the males clelivered naturally, we executed seven offspring daily from postnatal 1 d to 7 d, then sterile stripping hippocampus of the offspring, routine fixed ,chipped ,observed the ultra - structure change of hippocampus neurons synapses with the electron microscope. Results With the increase of age, the number of neurons of the hippocampns has no obvious change, but the number and volume of organelles in the hippocampus cells （ mitochondria, ER, etc. ） have gradually increased. The curvature of synaptic interface and post - synaptic density are rapidly increasing day by day （ all P 〈 0. 05 ） ; synaptic cleft gradually increased, from 3 to 7 days increased very rapidly （ all P 〈 0. 05 ）. Conclusion The changes of hippocampus neurons and synapses ultra - structure may be the foundation of rapidly develop of learning and memory ability in early born mice.

关 键 词：仔鼠 海马 突触 超微结构 

分 类 号：R338[医药卫生—人体生理学]