噬菌蛭弧菌颗粒剂制备条件的优化  

Optimization of preparation conditions of Bdellovibrio bacteriovorus granules

作　　者：张小能[1] 杨先乐[1] 曹海鹏[1] 胡鲲[1] 安健[1] 黄志华[1] 

机构地区：[1]上海海洋大学国家水生动物病原库,上海201306

出　　处：《上海海洋大学学报》2010年第6期778-786,共9页Journal of Shanghai Ocean University

基　　金：现代农业产业技术体系建设专项资金(NYCYTX-49-17);鲫鱼细菌性败血症等病害的综合防控技术研究与示范(200803013);对虾现代产业技术体系研究与建立(nyhyzx07-042)

摘　　要：试验采用海藻酸钠包埋法对噬菌蛭弧菌(Bdellovibrio bacteriovorus)进行固定化,制备得到噬菌蛭弧菌颗粒。以噬菌蛭弧菌活菌数为考核指标,优化噬菌蛭弧菌固定化条件。通过单因素试验和正交试验,评价了海藻酸钠浓度、氯化钙浓度、蛭弧菌菌液与海藻酸钠胶体溶液的比例(V/V)、玉米油浓度、包埋温度、搅拌时间、搅拌速度和交联时间对噬菌蛭弧菌固定化的影响。结果表明,作为载体配方优化因素的海藻酸钠浓度和玉米油浓度对活菌数具有显著性影响,而氯化钙浓度和蛭弧菌菌液与海藻酸钠胶体溶液的比例(V/V)影响不显著;作为过程优化因素的温度和搅拌速度对活菌数具有显著性影响,而搅拌时间和交联时间影响不显著。在海藻酸钠浓度1.5%、氯化钙浓度6.5%、蛭弧菌菌液与海藻酸钠胶体溶液的比例(V/V)50%、玉米油浓度4.5%、包埋温度47.5℃、搅拌时间40min、搅拌速度450r/min和交联时间36h的条件下,制得的固定化蛭弧菌颗粒活菌数对数值达到7.51。本方法经济、安全、保存效能好,适合在水产养殖中应用。Different conditions of Bdellovibrio bacteriovorus immobilization were investigated in this study,and the optimal conditions of Bdellovibrio bacteriovorus immobilization were confirmed by adopting sodium alginate as embedding medium,survived bacterium number as indices.Single-factor and orthogonal experiments were employed to evaluate the influences of sodium alginate concentration,calcium chloride concentration,the ratio of Bdellovibrio bacteriovorus solution to sodium alginate colloidal solution （V /V） ,corn oil concentration,encapsulating temperature,stirring time,stirring rate and cross-linking time on the immobilization of Bdellovibrio bacteriovorus.The results showed that sodium alginate concentration and corn oil concentration had significant influence on survival bacterium number,while calcium chloride concentration and the ratio of Bdellovibrio bacteriovorus solution to sodium alginate colloidal solution（V /V） were not in the orthogonal test for optimum carrier formula of immobilization.Furthermore,encapsulating temperature and stirring rate had significant influence on survival bacterium number while stirring time and crosslinking time were not in the orthogonal test for optimum parameters of immobilization.The optimal immobilization conditions were as follows： sodium alginate concentration of 1.5% ,calcium chloride concentration of 6.5% ,the ratio of Bdellovibrio bacteriovorus solution to sodium alginate colloidal solution（V /V） of 50% ,corn oil concentration of 4.5% ,encapsulating temperature at 47.5 ℃ ,stirring time for 40 min,stirring rate for 450 r /min and crosslinking time for 36 h.Under the above conditions,the logarithm of survival bacterium number of granules was up to 7.51.Therefore,the results demonstrated that the method with the properties of economy,security and good preservation has a promising future in aquaculture.

关 键 词：噬菌蛭弧菌 颗粒剂 制备 优化 

分 类 号：S963.1[农业科学—水产养殖]