CIMMYT 273个小麦品种抗病基因Lr34/Yr18/Pm38的分子标记检测  

Molecular Characterization of Lr34/Yr18/Pm38 in 273 CIMMYT Wheat Cultivars and Lines Using Functional Markers

作　　者：伍玲[1,2,3] 夏先春[2] 朱华忠[3] 李式昭[3] 郑有良[1] 何中虎[2,4] 

机构地区：[1]四川农业大学小麦研究所,成都611130 [2]中因农业科学院作物科学研究所/国家小麦改良中心,北京100081 [3]四川省农业科学院作物研究所,成都610066 [4]国际玉米与小麦改良中心北京办事处,北京100081

出　　处：《中国农业科学》2010年第22期4553-4561,共9页Scientia Agricultura Sinica

基　　金：“西部之光”人才培养计划、四川省农作物育种攻关项目(2006YZGG-3)

摘　　要：【目的】明确CIMMYT观察圃273个小麦品种(系)在慢病基因Lr34/Yr18/Pm38位点的等位变异类型及其对条锈病、叶锈病和白粉病的抗性,进一步验证抗病基因功能标记的有效性。【方法】利用Lr34/Yr18/Pm38紧密连锁的STS标记csLV34和基于该基因第11外显子(exon11)等位变异开发的5对功能标记cssfr1-cssfr5检测新引进的273个CIMMYT小麦品种(系),同时在成都和北京分别对其进行田间条锈病和白粉病的抗性鉴定,并结合CIMMYT的田间条锈病和叶锈病抗性鉴定结果进行分析。【结果】STS标记csLV34与5对功能标记cssfr1-cssfr5检测结果的一致性为96.7%;在273份CIMMYT材料中有43份材料含有Lr34/Yr18/Pm38,在不同地点对条锈病、叶锈病和白粉病具有不同程度的抗病性。【结论】功能标记cssfr1-cssfr5可准确鉴定Lr34/Yr18/Pm38位点exon11中的等位变异,cssfr3、cssfr4、cssfr5可用于含有Lr34/Yr18/Pm38材料杂交后代的分子标记辅助选择。【Objective】 The objectives of this study were to characterize the allelic variation at Lr34/Yr18/Pm38 locus in 273 CIMMYT wheat cultivars （lines）, evaluate their resistance against stripe rust, leaf rust and powdery mildew, and validate the functional markers for the locus. 【Method】 A total of 273 CIMMYT wheat genotypes were tested using a STS marker csLV34 and 5 functional markers cssfr1-cssfr5 which were developed based on a 3-bp deletion in the exon 11 of Lr34/Yr18/Pm38 gene, and were tested for stripe rust resistance in fields in Chengdu and CIMMYT, respectively, leaf rust in CIMMYT, and powdery mildew in Beijing. 【Result】 The results tested by the STS marker csLV34 showed a 96.7% identity to those of cssfr1-cssfr5. Of the 273 lines, 43 were characterized to have Lr34/Yr18/Pm38 resistance gene, with partial resistance against stripe rust, leaf rust and powdery mildew in field across different environments. 【Conclusion】 The 5 functional markers （cssfr1-cssfr5） could be used to detect the allelic variants in Lr34/Yr18/Pm38 locus, and 43 CIMMYT lines with Lr34/Yr18/Pm38 may be used in wheat breeding targeting for stripe rust, leaf rust and powdery mildew resistance with marker-assisted selection of cssfr3, cssfr4 and cssfr5.

关 键 词：普通小麦 Lr34/Yr18/Pm38 功能标记 慢病性 

分 类 号：S512.1[农业科学—作物学]