组合生防菌与解盐促生菌复配对棉花的促生效应  

Effects of Combination of Biocontrol Bacteria Complex with Salt Stress-Relieving Strain on Promotion of Cotton Growth

作　　者：赵雅峰[1] 陶晶[2] 武占省[1,2] 刘燕[2] 李春[2] 

机构地区：[1]石河子大学农学院/新疆兵团化工绿色过程重点实验室,新疆石河子832003 [2]北京理工大学生命学院,北京100081

出　　处：《中国农业科学》2010年第22期4754-4760,共7页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(20904033);新疆维吾尔自治区高新技术重点项目(200811108)

摘　　要：【目的】研究多功能菌剂BCL-Rs对盐胁迫和立枯丝核菌(Rhizoctonia solani)危害下棉花生长的影响。【方法】组合生防菌枯草芽孢杆菌(Bacillus subtilis)BCL-8与解盐促生菌产酸克雷伯氏菌(Klebsiella oxytoca)Rs-5复配获得BCL-Rs,通过盆栽、小区和大田试验对BCL-Rs促进棉花生长的功能进行验证。【结果】BCL-Rs对立枯丝核菌有很好的抑菌活性,并且能够有效提高棉花的耐盐性。盆栽试验和小区试验结果表明,BCL-Rs对棉花立枯病的防治效果分别达到了55.37%和51.06%,在盐胁迫条件下使棉苗干重分别提高了40.85%和25.81%,具有显著的防病促生和缓解盐胁迫的效能。棉花大田试验结果表明,BCL-Rs包衣处理及滴灌处理对棉花苗期生长有明显的促进作用,可显著提高棉花植株的鲜重、干重和株高,增大植株的叶面积,较常规处理增产10.51%。【结论】在BCL-8的防病性能与Rs-5的解盐促生功能的协同效应下,BCL-Rs能够更有效地防治盐胁迫条件下的棉花立枯丝核菌造成的危害。【Objective】 Effects of multi-functional bacterial mixture BCL-Rs on promotion of cotton growth under salt stress and Rhizoctonia solani damping-off were studied. 【Method】 BCL-Rs were obtained by combining biocontrol bacteria complex BCL-8 with salt stress-relieving strain Klebsiella oxytoca Rs-5. The function of BCL-Rs on cotton growth was investigated in pot tests, plot experiments and field trials. 【Result】 The BCL-Rs had an obvious inhibitory activity to Rhizoctonia solani, and BCL-Rs-treated cottons showed a good performance of relieving salt stress, and significant resistance to damping-off caused by Rhizoctonia solani. Compared with the control, the control efficacy of damping-off on cotton reached up to 55.37% and 51.06% in pot and plot experiments, respectively. And the dry weight of cotton seedlings increased by 40.85% and 25.81% under the salt stress conditions, respectively. The results of cotton field trials showed that the growth of cotton seedlings was promoted by coating and drip irrigation with BCL-Rs. Physiological characters of cotton, including plant fresh weight, dry weight, plant height, and leaf area increased significantly. The production of cotton increased by 10.51% compared with the conventional treatment. 【Conclusion】 Cotton damping-off caused by Rhizoctonia solani was significantly suppressed by the bacterial mixture BCL-Rs even under the salt stress conditions, indicating a synergistic effect of biocontrol bacteria BCL-8 and salt stress-relieving strain Rs-5.

关 键 词：棉花 多功能菌剂 枯草芽孢杆菌 立枯丝核菌 解盐 

分 类 号：S562[农业科学—作物学]