离子液体分散液相微萃取-高效液相色谱法测定番茄中的氨基甲酸酯类农药  

Determination of Carbamate Pesticides in Tomato Samples Using Ionic Liquid-based Dispersion Liquid Phase Microextraction Coupled with High Performance Liquid Chromatography

作　　者：郝家勇[1] 罗小玲[1] 唐宗贵[1] 王东健[1] 

机构地区：[1]新疆农垦科学院检测中心,新疆石河子832000

出　　处：《分析测试学报》2010年第11期1169-1172,1179,共5页Journal of Instrumental Analysis

基　　金：国家科技支撑计划资助项目(2007BAC20B00)

摘　　要：将离子液体1-丁基-3-甲基咪唑六氟磷酸盐([BMIm][PF6])作为萃取剂,采用分散液相微萃取-高效液相色谱法分析番茄样品中的4种氨基甲酸酯类农药残留,并考察了不同缓冲溶液浓度、pH值及萃取时间等因素对分散液相微萃取效果的影响。在优化实验条件下,该方法对涕灭威、克百威、甲萘威、异丙威的富集倍数分别为317、430、545、625,且具有良好的线性范围(0.01～10mg/L)和较低的检出限(0.12～0.43μg/L),对涕灭威、克百威、甲萘威、异丙威测定5次的相对标准偏差为5.3%～5.5%,用于示范区采集的番茄样品分析,平均加标回收率为75%～120%。研究表明[BMIm][PF6]可有效地萃取番茄中的氨基甲酸酯类农药残留,具有萃取效率高、灵敏度高、操作简单、绿色环保等优点,可以满足番茄样品中氨基甲酸酯类农药残留的检测要求。A new method using ionic liquid l-butyl-3-methylimidazolium hexafluorophosphate （ [ BMhn] [ PF61 ） for the determination of four carbamate pesticides in tomato samples was developed based on dispersion liquid phase microextraction coupled with high performance liquid chromatography. Under the optimized chromatographic conditions, the influences of extraction parameters including buffer solution type, pH value and extraction time on extraction efficiency were investigated. Under the optimal conditions, the enrichment folds of aldicarb, carbofuran, carbaryl and isoprocarb were 317, 430, 545 and 625, respectively. The method showed a good linearity in the range of 0. 01 - 10 mg/L for four pesticides and their detection limits were in the range of O. 12 -0.43 μg/L. The rela- tive standard deviations （ RSDs, n = 5） were between 5.3% and 5.5% . The average spiked recoveries for the pesticides in tomato samples collected from demonstration area were in the range of 75% - 120%. The results showed that the [ BMhn] [PF6] could be used for concentrating the carbaInate pesticides effectively in tomato. With the advantages of high extraction efficiency, good sensitivity, simplicity and green, the method could meet the requirement for determination of carbamate pesticide residues in tomato samples.

关 键 词：离子液体 氨基甲酸酯类农药 分散液相微萃取 

分 类 号：O657.72[理学—分析化学] F767.2[理学—化学]