核固红荧光猝灭法测定壳聚糖含量  

Fluorescence Quenching Method for the Determination of Chitosan with Nuclear Fast Red

作　　者：穆志英[1] 董存智[1] 

机构地区：[1]淮北师范大学化学与材料科学学院,安徽淮北235000

出　　处：《分析测试学报》2010年第11期1221-1224,共4页Journal of Instrumental Analysis

摘　　要：在pH3.8的醋酸-醋酸钠缓冲介质中,壳聚糖对核固红的荧光强度有明显的猝灭作用,且在一定浓度范围内,壳聚糖的质量浓度与荧光猝灭程度呈线性关系,据此建立了荧光猝灭法测定壳聚糖含量的新方法。方法的线性范围为0.008～2.5mg/L,检出限为5.4μg/L。对猝灭机理进行了初步探讨,并将方法用于保健食品中壳聚糖含量的测定,回收率高,结果满意。A sensitive fluorescence quenching method was developed for the determination of chitosan based on its notable quenching effect on the fluorescence of nuclear fast red in pH 3.8 NaAc - HAc buffer. The fluorescence quenched intensity of nuclear fast red was linear with chitosan in a certain concentration range. Under the optimum conditions, the calibration curve was linear in the range of 0. 008 - 2. 5 mg/L with a detection limit of 5.4 μg/L. The quenching mechanism was discussed. The method was applied in the determination of chitosan in different health food samples with satisfactory results.

关 键 词：核固红 壳聚糖 荧光猝灭 

分 类 号：O657.3[理学—分析化学]