蛋鸡J亚群禽白血病病毒HLJ09MDJ-1株的分离鉴定  

Isolation and Identification of Avian Leukosis Virus Subgroup J HJL09MDJ-1 Strain in Layers

作　　者：潘伟[1] 高玉龙[1] 秦立廷[1] 祁小乐[1] 高宏雷[1] 孙芬芬[1] 王永强[1] 王笑梅[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,禽传染病研究室,黑龙江哈尔滨150001

出　　处：《动物医学进展》2010年第11期1-5,共5页Progress In Veterinary Medicine

基　　金：现代农业肉鸡产业技术体系建设项目(nycytx-42-G3-01);公益性行业(农业)科研专项项目(200803020-XX03)

摘　　要：为了解蛋鸡中禽白血病当前流行毒株类型及其gp85基因分子特征,在临床症状和病理组织学观察的基础上,采用ELISA抗原检测、特异性PCR以及亚群特异性免疫荧光试验(IFA),证实从黑龙江省某鸡场送检蛋鸡中分离到1株J亚群禽白血病病毒(ALV-J),命名为HLJ09 MDJ-1;克隆了其gp85基因,并进行了序列分析比较。结果表明,该分离株的gp85基因与国内外其他ALV-J毒株的核苷酸同源性为88.2%～98.2%。其中,HLJ09 MDJ-1与英国原型株HPRS-103的同源性最高(98.2%),与美国株ADOL-7501的同源性最低(88.2%),表明HLJ09 MDJ-1的gp85基因与HPRS-103有较近的亲缘关系。本研究从蛋鸡中分离到ALV-J毒株,为研究ALV-J宿主嗜性改变和ALV-J对蛋鸡致病机制提供了素材。To find out the current prevalent ALV strain in layers and its molecular characteristics,a strain of subgroup J avian leukosis virus（ALV-J）,named HLJ09MDJ-1,was isolated and identified by clinical symptoms and histopathological observation,PCR amplication and immunofluorescence assay special to ALV-J from layers suspected with ALVs infection in a farm of Helongjiang province in China.Moreover,the gp85 gene of HLJ09MDJ-1 was cloned and analyzed with other ALV-J strains published in GenBank.The results showed that the gp85 gene of HLJ09MDJ-1 shared 88.2%-98.2% homology in the level of nucleotide acid sequences with other ALV-J strains,among which,the highest homology（98.2%） with HPRS-103 and the lowest homology（88.2%） with ADOL-7501.Overall,these data suggest a close relativity between the gp85 genes of HLJ09MDJ-1 and HPRS-103.In conclusion,the HLJ09MDJ-1 strain isolated from egg-type chickens may contribute to the researches on the changes of ALV-J host range and pathogenicity to egg-type chickens.

关 键 词：蛋鸡 J-亚群禽白血病病毒 分离鉴定 GP85基因 遗传进化分析 

分 类 号：S852.659.3[农业科学—基础兽医学]