乙肝表面抗体和抗人红细胞单克隆抗体的F(ab′)_2偶联与鉴定  

Identification and Conjugation of F(ab′)_2 Segment of Human Erythrocyte McAb and Hbs Ab

作　　者：王云龙[1,2] 李兆学[1] 李恒思 李玉林 李忠信[1] 孙新城[3] 

机构地区：[1]河南工业大学,河南郑州450001 [2]河南省生物工程技术研究中心,河南郑州450001 [3]郑州大学,河南郑州450001

出　　处：《动物医学进展》2010年第11期30-33,共4页Progress In Veterinary Medicine

基　　金："十一五"国家科技支撑计划重点项目(2006BAF07B01)

摘　　要：制备人红细胞非凝集型单克隆抗体,形成双功能抗体试剂,用于临床诊断。用人O型红细胞膜蛋白作为抗原免疫Balb/c小鼠,采用淋巴细胞杂交瘤技术进行细胞融合,制备非凝集型单克隆抗体,通过化学偶联法将非凝集性单克隆抗体和乙肝表面抗体的F(ab′)2片段偶联,制备出双功能抗体,用直接凝集法检测乙肝表面抗原。获得5株分泌非凝集型杂交瘤细胞,利用戊二醛一步法制备出双功能抗体,形成全血凝集检测试剂,并进行了初步鉴定。Non-agglutinating McAb of human RBC was produced.Then,bispecific antibody reagent was prepared for clinical diagnosis.The membrane protein of type O human RBC was used as antigen to immunize the Balb/c mice.Non-agglutinating human RBC McAb was prepared through cell fusion with lymphocyte hybridoma technique.The bispecific antibody reagent is produced through chemical cross-linking of F（abˊ）2 segment of non-agglutinating McAb and HBV surface antibody.The reagent can be used to diagnose HBV surface antigen through direct agglutination reaction.Five non-agglutinating hybridoma cell lines were obtained,and the bispecific antibody was made by one-step glutaraldehyde method.Furthermore,the whole-blood agglutinating test reagent was formed and primary identification experiment was carried out.

关 键 词：人红细胞 单克隆抗体 双功能抗体 全血凝集 

分 类 号：R392.11[医药卫生—免疫学]