滋阴益气活血组方对STZ糖尿病模型大鼠糖代谢的影响  

Effect of Yin-enriching,Qi-boosting and Blood-quickening Compound Formula on Glycometabolism in Diabetic Rats Induced by STZ

作　　者：李静[1] 刘德义[1] 程昌勇[1] 

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100

出　　处：《动物医学进展》2010年第11期47-50,共4页Progress In Veterinary Medicine

基　　金：安徽科技学院引进人才专项基金资助项目(ZRC2007139)

摘　　要：为探讨滋阴益气活血组方对链脲佐菌素(STZ)糖尿病大鼠糖代谢的影响,用STZ尾静脉注射45 mg/kg诱导建立糖尿病大鼠模型,将符合糖尿病诊断标准的大鼠,随机分为模型对照组(n=15)和组方治疗组(n=15)。同时设立正常对照组(n=20)。组方治疗组灌胃滋阴益气活血组方水煎液10 mL/kg.d,模型对照组和正常对照组灌胃相当剂量的生理盐水,灌胃期为56 d。灌胃第14、28、42、56天眶后静脉丛采血,离心制备血清以测血糖(GLU),第56天测血清糖化血清蛋白(GSP),处死大鼠取肝脏测肝糖原的含量。用组方治疗组水煎液连续灌胃8周后,该组大鼠的体重相对于模型对照组处于上升趋势;第56天时,血糖极显著低于模型对照组(P<0.01),基本接近正常水平;糖化血清蛋白含量极显著低于模型对照组(P<0.01),肝糖原含量极显著高于模型对照组(P<0.01)。滋阴益气活血组方能够改善和纠正糖尿病大鼠糖代谢的异常,对临床研究中药治疗糖尿病具有指导意义。The aim of the research is to study effects of Yin-enriching,Qi-boosting and Blood-quickening compound formula（YQBCF） on glycometabolism in diabetic rats induced by STZ.45 mg/kg STZ was injected through caudal vein to establish diabetic Ⅱ rat models,and the rats were randomly divided into YQBCF treatment group（n=15）,model control group（n=15）,and normal control group（n=20）.The rats in treatment group was given YQBCF water solution[10 mL/（kg·d）] by stomach perfusion,the rats in model control group and normal control group were given the same amount of distilled water.The perfusion period is 56 days.After 14,28,42,56 days,the blood was taken in every rat through orbital venous plexus,and the serum was separated to measure glucose（GLU）.On the 56th day,the glycosylated serum protein（GSP） was examined,then the rats were killed and the liver glycogen was examined.The body weight of rats in YQBCF treatment group raised during the trial period compared with that in the model control group.The levels of glucose（GLU）,liver glycogen and glycosylated serum protein（GSP）showed an improving tendency in YQBCF treatment group.On the 56th day,glucose and GSP in rats of YQBCF treatment group decreased significantly compared with that of the model control group（P0.01）,which was near the normal levels.Liver glycogen in rats of YQBCF treatment group significantly increased（P0.01） compared with that of the model control group.The data demonstrated that YQBCF can obviously improve glycometabolism and the symptoms in diabetic rats,which will provide the value for the clinical treatment of diabetes with Chinese traditional medicine.

关 键 词：糖尿病 滋阴益气活血 链脲佐菌素 血糖 肝糖原 糖化血清蛋白 

分 类 号：R587.1[医药卫生—内分泌] S853.73[医药卫生—内科学]