秦公散对LPS活化的内皮细胞表达黏附分子和分泌一氧化氮的影响  

The Effects of Qingongsan on the Adhesion Molecule Expressing of Endothelial Cell Activated by LPS

作　　者：李培锋[1] 李成应[1] 希日莫 关红[1] 李利平 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [2]内蒙古兽药监察所,内蒙古呼和浩特010010 [3]呼和浩特兽药监督管理所,内蒙古呼和浩特010020

出　　处：《动物医学进展》2010年第11期67-70,共4页Progress In Veterinary Medicine

基　　金：呼和浩特市科技局项目(2005-农-7)

摘　　要：为阐明秦公散的抗炎作用机制,本试验采用流式细胞术检测了LPS刺激的内皮细胞黏附分子E-选择素和ICAM-1的表达,用一氧化氮(NO)测定试剂盒检测LPS刺激的内皮细胞培养上清液中NO的含量。结果表明,LPS刺激的内皮细胞可显著提高E-选择素和ICAM-1的表达(P<0.01),显著提高培养上清液中NO的含量(P<0.05);用秦公散及其含药血清处理后则明显抑制LPS诱导的E-选择素、ICAM-1的表达和NO分泌(P<0.05)。表明秦公散可通过抑制黏附分子的表达及NO分泌量发挥抗炎作用。To state the Anti-inflammatory effects mechanism of Qingongsan,the flow cytometry was adopted to detect the expression of E-selectin and ICAM-1 in the endothelial cell activated by LPS and the NO determining kit was adopted to determine the concentration of NO in cultivation supernatant.Results： the human umbilical vein endothelial cells were cultured with LPS together can remarkably increase the expression of E-selectin and ICAM-1（P0.01）,treated by crude extract of Qingongsan and the serum containing drugs can inhibit the expression of E-selectin,ICAM-1 and increase secretary of NO induced by LPS（P0.05）.Qingongsan can educe its anti-inflammation function though inhabiting the expression of adhesion molecule and excessive secretion of NO.

关 键 词：细菌内毒素 内皮细胞 E-选择素 细胞间黏附分子-1 一氧化氮 

分 类 号：S859.7[农业科学—临床兽医学]