细菌人工染色体荧光原位杂交技术的应用  

Application of Bacterial Artificial Chromosome Fluorescence in Situ Hybridization

作　　者：娜日苏[1] 陈献伟[1,2] 冯宝刚[1,3] 雷娜[1,4] 赵瑞杰[1,5] 关伟军[1] 马月辉[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]新疆石河子大学生命科学学院,新疆石河子832003 [3]广西大学动物科学技术学院,广西南宁530004 [4]山西农业大学动物科技学院,山西太谷030801 [5]西北农林科技大学动物医学院,陕西杨陵712100

出　　处：《动物医学进展》2010年第11期95-99,共5页Progress In Veterinary Medicine

基　　金：国家"863"计划重大专项资助项目(2006AA10Z198;2007AA10Z170);国家科技支撑计划项目(2006BAD13B08;2008BADB2B01);农业部转基因项目(2008ZX08009-003)

摘　　要：目前,覆盖人类、植物、畜禽、微生物等100多种物种全基因组的细菌人工染色体文库在不同时间、不同群体中相继产生,为物种基因资源的保存、基因组学和后基因组学的研究提供了可靠的材料。细菌人工染色体与荧光原位杂交技术的结合能够将物种的大片段基因组DNA杂交在染色体上,确定基因或标记物在染色体上的物理位置,从而成为细胞和分子生物学领域中必不可少的工具。论文对细菌人工染色体荧光原位杂交技术在染色体结构与核型分析、疾病与肿瘤病原学研究、基因和标记物的定位与细胞遗传图谱绘制、基因组比较作图及物种进化中的应用和发展进行了综述。Bacterial articial chromosome libraries for more than 100 species containing human,plant,live-stock,poultry and microorganism have been constructed at different time and in different populations,which contribute on supplying reliable materials for genomic resource conservation,genomics and post-genomics research,and genome project achievement.And the bacterial artificial chromosome vector performed to fluorescence in situ hybridization can hybrid a large genomic DNA on chromosome and define the physical mapping of genes or marker,becoming the essencial tools for cellular and molecular research.This paper overviewed the development and application of the BAC-FISH technology in recent years,and mainly focused on the areas of the karyotype analysis and chromosome identification,chromosomal abnormalities,etiology research,physical mapping of gene and marker,cytogenetic mapping,comaparative genome mapping,and species evolution research.

关 键 词：细菌 人工染色体 荧光原位杂交 应用 

分 类 号：S854.43[农业科学—临床兽医学]