电喷雾质谱法研究N-苯甲酰基-脱氢枞胺-7-酮与DNA的相互作用  

Study on the Interaction Between N-benzoyl-Dehydroabitylamine-7-one and DNA by ESI/MS Analysis

作　　者：管小虹[1] 邓慧敏[1] 林中祥[2] 

机构地区：[1]中山大学测试中心,广东广州510275 [2]南京林业大学化学工程学院,江苏南京210037

出　　处：《中山大学学报（自然科学版）》2010年第6期74-77,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(30771688;30871989)

摘　　要：采用电喷雾质谱(ESI-MS)法研究了N-苯甲酰基-脱氢枞胺-7-酮与4种DNA—dC6,dT6,dA6,d(AT)3的相互作用,考察了N-苯甲酰基-脱氢枞胺-7-酮与DNA形成复合物的电喷雾质谱行为及N-苯甲酰基-脱氢枞胺-7-酮-DNA复合物-4价离子的二级质谱(MS/MS)行为。结果表明,N-苯甲酰基-脱氢枞胺-7-酮与4种DNA均可形成非共价复合物,且复合物离子主要以-4价形式存在;N-苯甲酰基-脱氢枞胺-7-酮与4种不同的DNA形成的-4价复合物离子均在碰撞能(CE)约20%时发生裂解,并且具有大致相同的裂解方式。The interactions between N-benzoyl-dehydroabietylamine-7-one（NBDO） and dC6,dT6,dA6 and d（AT）3 were studied by ESI-MS analysis.The [NBDO＋DNA-4H]4-complexes ions were investigated by MS/MS method.The results showed that the NBDO could formed non-covalent complexes with dC6,dT6,dA6,d（AT）3,and the complexes ions were mainly charged-4.The [NBDO＋DNA-4H]4-ions fragmented at approximately 20% collision energy and all [NBDO＋DNA-4H]4-ions had similar fragmentation mode.

关 键 词：电喷雾电离质谱 N-苯甲酰基-脱氢枞胺-7-酮 DNA 相互作用 MS/MS 

分 类 号：O657.63[理学—分析化学]