红腺忍冬的质量研究  

Study on the Quality Cotrol of Lonicera hypoglauca Miq.

作　　者：彭维[1] 黄琳[2] 关倩怡[1] 林青[2] 梁峰[1] 苏薇薇[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]广州白云山和记黄埔中药有限公司,广东广州510515

出　　处：《中山大学学报（自然科学版）》2010年第6期142-144,共3页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省科技计划资助项目(2010B050100013)

摘　　要：采用薄层色谱法对红腺忍冬进行了定性鉴别;用高效液相色谱法同时测定绿原酸和咖啡酸的含量,色谱柱为Dikma PLATISIL ODS(250 mm×4.6 mm,5μm),流动相为乙腈-0.4%(w)磷酸溶液(pH≈2.5)(12:88),检测波长327 nm。结果显示绿原酸加样回收率为99.6%、RSD为2.16%,咖啡酸加样回收率为101.1%、RSD为3.26%。该方法可用于红腺忍冬药材的质量控制。In this paper,Lonicera hypoglauca Miq.was identified by TLC,chlorogenic acid and caffeic acid were determined by HPLC.The chromatographic column was Dikma PLATISIL ODS（250 ×4.6 mm,5 μm）,gradient elution with acetonitrile and 0.4% phosphoric acid（pH≈2.5）（12∶88）was applied and the detection wavelength was 327 nm.The recovery of chlorogenic acid was 99.6% with RSD 2.16%,and the recovery of caffeic acid was 101.1% with RSD 3.26%.The established method can be used for quality control of Lonicera hypoglauca Miq..

关 键 词：红腺忍冬 绿原酸 咖啡酸 薄层色谱法 高效液相色谱法 

分 类 号：R961[医药卫生—药理学]