玉米制种田父本去杂分子标记辅助技术研究  

Research on the Molecular Marker Assistant Techniques of Male Parent in Hybrid Corn Seed Cropland

作　　者：吴改荣[1] 段云佳[1] 宋强[1] 李莎弥[1] 高文伟[1] 

机构地区：[1]新疆农业大学,乌鲁木齐830052

出　　处：《新疆农业科学》2010年第10期1981-1985,共5页Xinjiang Agricultural Sciences

基　　金：新疆农业大学自治区重点产业紧缺人才专业第一批立项大学生创新项目(37);新疆农业大学农业生物技术重点实验室项目

摘　　要：【目的】以玉米制种田父本为研究对象,建立真实性鉴定试剂盒。【方法】采用优化的SSR分子标记技术,对制种田父本进行实证分析。【结果】利用SSR标记技术筛选出玉米的特异性引物,结合单籽粒DNA快速提取方法,建立了种子鉴定试剂盒。【结论】应用此试剂盒可快速、有效地鉴定父本的种子纯度和真实性,为田间间苗、定苗提供依据;为制种田父本去除杂株提供技术支撑。【Objective】The objective of this study was to establish the authentic kits for identification using male parent of hybrid corn seed cropland as test materials.【Method】The positive analysis was conducted to study the male parent in hybrid corn seed cropland with optimal SSR molecular markers.【Result】 The application of this kit can be quickly and effectively identify the male parent seed purity and authenticity.【Conclusion】 SSR markers were screened using specific primers of maize,combined with DNA rapid extraction method of a single grain,seed identification kit was set up.Experiments show that this kit can quickly and effectively identify paternal authenticity.It provided the basis for thinning out seedlings and final singling,and provided the new techniques for weeding out the weedplants.

关 键 词：玉米 分子标记 真实性 品种纯度 

分 类 号：S513.035.2[农业科学—作物学]