牛血红蛋白与双十二烷基二甲基溴化铵相互作用的光谱研究(英文)  

Study on the interaction between hemoglobin and didodecyldimethylammonium bromide by spectroscopic technique

作　　者：高筱玲[1] 田燕妮[2] 

机构地区：[1]山西省分析测试中心,山西太原030006 [2]山西大学分子科学研究所,山西太原030006

出　　处：《化学研究与应用》2010年第11期1361-1365,共5页Chemical Research and Application

基　　金：山西省自然科学基金资助项目(2006011016)

摘　　要：运用荧光光谱、紫外-可见吸收光谱和圆二色谱法研究了双十二烷基二甲基溴化铵(DDAB)与牛血红蛋白(Hb)的相互作用。从紫外-可见吸收光谱观察到,随着DDAB的浓度增大,Hb在406nm处的特征吸收峰强度下降,且峰位蓝移,说明DDAB导致血红素辅基微观环境变化。由荧光光谱研究可以得出随着DDAB的浓度增大,Hb在340nm处的荧光强度逐渐增强,说明导致色氨酸荧光淬灭的血红素辅基与色氨酸的距离增大。由Scatchard方程计算了不同温度下该反应的表观结合常数、结合位点数及结合热力学参数,热力学参数的变化表明DDAB与Hb之间以疏水作用力为主。圆二色谱的研究进一步表明DDAB使Hb产生轻微的二级结构改变,α-螺旋含量增加.The interaction between hemoglobin （Hb） and didodecyldimethylammonium bromide （DDAB） was investigated by UVvis spectra,fluorescence spectra and circular dichroism spectra （CD）.With the addition of DDAB into the solution of Hb,the Soret absorption decreased gradually,the fluorescence intensity of Hb increased remarkably and α-helical content of Hb increased.According to Scatchard analysis,the binding constants of DDAB to Hb were obtained at different temperature.The parameters of thermodynamics,ΔH,ΔS and ΔG,indicated that the hydrophobic force should be mainly interaction of DDAB with Hb.

关 键 词：牛血红蛋白 荧光光谱 圆二色谱 热力学参数 

分 类 号：O657.3[理学—分析化学]