BMP-2在糖尿病肾病大鼠肾内小动脉中表达的意义  

Significance of BMP-2 expression at renal arteriole in diabetic nephropathy rats

作　　者：赵安菊[1] 黄颂敏[2] 欧三桃[2] 焦桂萍[1] 王里[1] 陈超[1] 

机构地区：[1]四川大学华西医院肾内科,成都640000 [2]贵州省人民医院干医科,贵阳550001

出　　处：《重庆医学》2010年第22期3004-3006,I0001,共4页Chongqing medicine

基　　金：贵州省科技厅基金资助项目(黔科合J字[2008]2299号)

摘　　要：目的观察骨形态蛋白2(BMP-2)在糖尿病肾病(DN)大鼠肾实质内小动脉上的表达及其变化规律,探讨其对糖尿病肾病血管病变的影响。方法设对照组(N组)和糖尿病肾病组(DN组),建立链脲佐菌素(STZ)诱导的DN大鼠模型。茜素红染色观察肾实质内小动脉周围钙盐沉积,并分别采用免疫组化及mRNA原位杂交法检测BMP-2在肾实质内小动脉上的蛋白表达及基因定位。结果 (1)茜素红染色DN组第4～12周时偶见肾内小动脉少许点状红色钙盐沉积,第24周时可见肾实质内小动脉及周围组织有较多红色点片状沉积,N组茜素红染色为阴性。(2)DN组肾实质内小动脉BMP-2蛋白及mRNA在4周时已有表达,随时间延长表达逐渐增强,各时间点均明显高于N组,N组各时间点表达差异无统计学意义。结论 (1)DN大鼠肾内小动脉钙化前即已有BMP-2的表达;(2)BMP-2可能是糖尿病血管钙化的重要转录因子及早期生物学标志之一;(3)肾内小动脉上BMP-2的表达变化可能参与DN进展。Objective To observe the expression of bone morphogenetic protein-2（BMP-2） in renal arteriole,and to explore its effect to vascular lesion of diabetic nephropathy.Methods Adult Sprague-Dawley male rats were divided into diabetic group and control group.Paraffin sections of renal tissue of rats at the same time points were stained by routine methods and alizarin red,immunohistochemistry,hybridization in situ.Results（1）A bit of calcium salt deposited in renal arteriole at 4 week and a lot calcium salt at 24 weeks.（2） From 4 week,the number of BMP-2 staining and mRNA in diabetic renal arteriole was significantly increased as compared with control group.Conclusion（1） BMP-2 has already expressed in renal arteriole before the formation of vascular calcification.（2） Before vascular calcification,BMP-2 in diabetic renal arteriole is significantly upregulated,which indicates that BMP-2 is one of key regulating factors of diabetic vascular calcification.（3）Alteration of the expression of BMP-2 in renal arteriole may indicate that it participates in the development of DN.

关 键 词：糖尿病肾病 血管钙化 骨形态蛋白2 

分 类 号：R587.2[医药卫生—内分泌] R543[医药卫生—内科学]