VEGF、HIF-1α在大鼠慢性哮喘模型中的表达及地塞米松的干预效应  

Expression of VEGF and HIF-1α in asthma chronic model and dexamethasone intervention

作　　者：刘鑫[1] 梅全慧[2] 刘希[2] 莫万勇 黄艳[1] 

机构地区：[1]南华大学附属第一医院中医科,湖南衡阳421001 [2]南华大学临床系,湖南衡阳421001 [3]湖南省邵阳市中心医院,422000

出　　处：《重庆医学》2010年第22期3013-3015,I0001,共4页Chongqing medicine

摘　　要：目的观察缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)在慢性哮喘大鼠中的表达水平,探讨其在慢性哮喘气道重塑中的作用。方法以卵清清蛋白(OVA)致敏及反复激发建立大鼠慢性哮喘模型,激发前30 min胃内灌注地塞米松1mg/kg,最后一次激发12 h内处死大鼠,常规病理切片观察大鼠肺内炎症情况、总气管壁厚度、气管内壁厚度及气道平滑肌厚度。免疫组化检测气道壁HIF-1α、VEGF蛋白的表达,RT-PCR观察大鼠肺部HIF-1α、VEGF mRNA的表达。结果慢性哮喘模型组大鼠气道支气管及血管周围大量炎症细胞聚集,总气管壁、气管内壁及气道平滑肌增厚,肺组织HIF-1α、VEGF mRNA及HIF-1α、VEGF蛋白的表达增高(P<0.01)。哮喘大鼠致敏前使用地塞米松后气道炎症较轻,减轻总气管壁、气管内壁及气道平滑肌增厚。各组大鼠肺组织内HIF-1α、VEGF蛋白的表达与气道壁厚度呈正相关。结论 HIF-1α、VEGF可能参与了慢性哮喘大鼠的气道重塑,早期使用地塞米松可以预防气道重塑。Objective To evaluate the relationship between VEGF,HIF-1α level and asthmatic chronic rats and to invetigate the effect of VEGF and HIF-1α in airway remodeling of chronic asthmatic rats.Methods Twenty-four SD rats were randomly divided into three groups;the asthma chronic model group,the dexamethasone group and the control group.The asthmatic model was established by sensitization and challenge with ovalbumin,and the rats in the dexamethasone group administrated dexamethasone by gastric gavage before the challenge.Immunohistochemistry and RT-PCR were used to measure the level of VEGF,HIF-1α protein and mRNA in the rats′ lung tissues.The thickness of the airway wall and its smooth muscle was estimated by a computerized digital image analyzer.Results（1）The thickness of the airway wall in the asthma chronic model group was higher than that in the other groups（P〈0.01）.（2）The expression of VEGF,HIF-1α protein and mRNA in the lung tissues of the asthma chronic model group was higher than that in the other groups（P〈0.01）.（3） VEGF,HIF-1α protein and mRNA expressions were positively correlated with airway wall thickness.Conclusion The expressions of VEGF,HIF-1α protein and mRNA are increased in the lung tissues of asthmatic chronic rats and positively correlated with the airway thickness.VEGF and HIF-1α may take part in the process of airway remodeling of asthma.

关 键 词：哮喘 气道重塑 缺氧诱导因子-1Α 血管内皮生长因子 地塞米松 

分 类 号：R562.25[医药卫生—呼吸系统] R977.11[医药卫生—内科学]