两种消除Klebsiella pneumoniae重组型质粒的方法  

Two methods for eliminating recombinant plasmids in Klebsiella pneumoniae

作　　者：王熙[1] 萨娜[1] 杨建国[1] 田平芳[1] 

机构地区：[1]北京化工大学北京市生物加工过程重点实验室,北京100029

出　　处：《北京化工大学学报（自然科学版）》2010年第6期112-115,共4页Journal of Beijing University of Chemical Technology(Natural Science Edition)

基　　金：国家自然科学基金(20876009)

摘　　要：对于工业发酵菌种肺炎克雷伯氏菌(Klebsiella pneumoniae),研究发现有两种消除其重组型质粒的有效方法,一种是连续传代培养,另一种是使用消除剂十二烷基硫酸钠(SDS)。对K.pneumoniae重组菌连续20代传代培养后,发现其质粒具有较高的消除率;而以0.2%SDS复合Ca2+处理K.pneumoniae重组菌,也能有效消除其重组型质粒,且该方法省却了反复的传代培养,能快速得到质粒消除菌,更具简便易操作性。消除了质粒的K.pneumoniae能再次接纳新的质粒,有效避免了因质粒不相容性带来的转化不成功,进而可用作宿主菌积累更多的生理性状。Good physiological performance of industrial microbes is crucial for successful bioprocessing. This generally requires the elimination of plasmids. In order to eliminate the recombinant plasmids in Klebsiella pneumoniae,two methods have been investigated,namely subculturing and sodium dodecyl sulphate （SDS） treatment. After 20 generations of subculturing,recombinant K. pneumoniae showed a high plasmid elimination ratio,and the recombinant strain treated by 0.2% SDS coupled with Ca2＋ also showed a high plasmid elimination ratio. After these treatments,the resulting plasmid-free strain was capable of uptaking new plasmids,which allows the host strain to accumulate other desirable physiological traits.

关 键 词：质粒 消除 肺炎克雷伯氏菌 重组 

分 类 号：Q933[生物学—微生物学]