阻滞和非阻滞品系小鼠早胚体外培养的比较  

Comparison of the in Vitro Development Potential of Early Embryos from Blocking and Non-blocking Strain Mice Respectively

作　　者：林翠英[1] 史河秀[2] 叶维建[1] 陈建明[1] 王世鄂[3] 

机构地区：[1]莆田学院医学院,福建莆田351100 [2]福建卫生职业技术学院基础医学部,福建福州350101 [3]福建医科大学人体解剖学与组织胚胎学系,福建福州350004

出　　处：《莆田学院学报》2010年第5期17-20,共4页Journal of putian University

基　　金：教育部回国人员启动基金项目(教外司[2005]383号);福建省高等学校新世纪优秀人才支持计划项目(NCETFJ-0601)

摘　　要：收集阻滞品系昆明(Kunming,KM)小鼠和非阻滞品系B6C3F1小鼠受精卵,以及KM小鼠和B6C3F1小鼠交叉交配所得受精卵,进行体外培养,研究阻滞品系小鼠早胚体外发育阻滞原因。结果表明,卵母细胞内在因素对子一代早胚体外发育的能力影响较大,即体外培养是否发生2-细胞阻滞与卵母细胞有关。The objective of this study was to investigate the cause for developmental arrest of early embryos in vitro from blocking strain mice by comparing the different development potential between Kunming（KM） and B6C3F1 1-cell embryos got from four kinds of hybrids（♀KM ×♂KM,♀B6C3F1×♂B6C3F1,♀KM×♂B6C3F1 and ♀B6C3F1×♂KM）.Reciprocal crosses between the eggs and the sperms of ＇blocking＇（KM）and ＇non-blocking＇（B6C3F1） strain mice show that the intracellular factor of the egg might determine whether the early embryo blocked at the 2-cell stage or continued developing beyond the 2-cell stage.

关 键 词：早胚 2-细胞阻滞 体外培养 小鼠 

分 类 号：Q959.837.04[生物学—动物学] Q954.44