金属酞菁仿生催化儿茶酚胺氧化性能研究及其用于肾上腺素浓度的光学检测(英文)  

Oxidation of Catecholamines Catalyzed by Metallophthalocyanines and Application to the Fiber Optic Biosensor for Adrenaline Concentration Detection

作　　者：李明田[1,2] 黄俊[3] 杨瑞嵩[1,2] 喻兰英[1,2] 周璇[3] 

机构地区：[1]材料腐蚀与防护四川省重点实验室,自贡643000 [2]四川理工学院材料与化学工程学院,自贡643000 [3]武汉理工大学光纤传感技术与信息处理教育部重点实验室,武汉430070

出　　处：《无机化学学报》2010年第11期2069-2076,共8页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.60877048);四川理工学院科技项目(2009xjkRL007)资助

摘　　要：采用电子吸收光谱法研究了5种金属酞菁MPcs（M=Mn（Ⅱ）,Fe（Ⅱ）,Ni（Ⅱ）,Cu（Ⅱ））仿生催化肾上腺素（Adrenaline,AD）和去甲肾上腺素（Noradrenaline,NA）2种儿茶酚胺的氧化性质,相应的氧化产物分别为三羟基-N-甲基-吲哚和三羟基-吲哚。用氧化产物的特征吸收峰强度评价金属酞菁的催化能力,实验表明,在最佳催化条件下,金属酞菁催化效率有以下顺序ηMnPc〉ηFePc〉ηNiPc〉ηCuPc〉ηCoPc。以酞菁锰仿生酶为催化剂,采用锁相放大技术构建了一种新型光纤生物传感器实现对肾上腺素浓度的测定,系统地研究了光纤肾上腺素传感器的性质：在2.0×10^-6-9.0×10^-5mol·L^-1范围,滞后相移φ与肾上腺素的浓度有较好的线性关系,检测下限为4.0×10^-7mol·L^-1,响应时间为10min,该传感器有良好地重复性和稳定性。The oxidation of catecholamines （adrenaline, AD; noradrenaline, NA） by oxygen using metallophthalocyanines [MPcs, M=Mn（Ⅱ）, Fe（Ⅱ）, Co（Ⅱ）, Ni（Ⅱ）, Cu（Ⅱ）] as the catalyst were studied by electronic absorption spectra, and the consequent products were trihydroxyl-N-methyl-indole and trihydroxyl-indole, respectively. The catalytic activities of the MPcs were evaluated by the absorbance ratios at the characteristic peak of the oxidation products. The results showed that MnPc had the best catalytic activity under the optimal conditions. The fiber optic AD biosensor based on MnPc catalysis and fluorescence quenching was fabricated and studied. The dissolved oxygen and AD content were evaluated by the phase delay φ. A linear relationship between φ and AD concentration was observed in the range from 2.0×10^-6 to 9.0×10^-5 mol·L^-1, and the detection limit was 4.0×10^-7 mol·L^-1. The results indicated that the fiber optic biosensor exhibited good reproducibility and stability.

关 键 词：金属酞菁 仿生酶 儿茶酚胺 催化氧化 光纤生物传感器 

分 类 号：O625.8[理学—有机化学] O643.361[理学—化学]