活血化瘀中药对脑出血大鼠神经功能和脑组织形态及血流灌注的影响  

Effects of the traditional Chinese medicine of activating blood circulation to dissipate blood stasis on neurological function,morphous and perfusion of brain tissue in intracerebral hemorrhage rats

作　　者：贺燕[1] 戴宏伟[2] 姬林[1] 甘洁[3] 余明[1] 于筠[2] 

机构地区：[1]山东中医药大学第二附属医院神经内科,济南250001 [2]山东中医药大学第二附属医院神经外科,济南250001 [3]山东中医药大学第二附属医院放射科,济南250001

出　　处：《临床神经病学杂志》2010年第5期363-366,共4页Journal of Clinical Neurology

基　　金：山东省中医药科技发展计划(2007-100)

摘　　要：目的探讨活血化瘀中药(抵挡丸)对脑出血大鼠神经功能和脑组织形态及血流灌注的影响。方法 50只雄性Wistar大鼠随机分为正常对照组(5只)、假手术组(5只)、脑出血模型组(模型组,20只)和抵挡丸治疗组(治疗组,20只);后两组又分别分为术后12h、24h、72h、7d4个时间点亚组。采用自体血尾状核注入法制备大鼠脑出血模型。治疗组大鼠予以0.08g/L抵挡丸3ml灌胃治疗,每日2次。应用CT灌注对血肿周围局部脑血流量(rCBF)、局部脑血容量(rCBV)及平均通过时间(MTT)进行定量测量,同时进行Be-derson评分与脑组织病理学检查。结果模型组和治疗组Bederson评分显著高于正常对照组和假手术组(均P<0.05);术后7d时治疗组Bederson评分明显低于模型组(P<0.05)。治疗组各时间点病理损害均较模型组轻。与正常对照组和假手术组比较,模型组和治疗组的rCBF、rCBV均显著降低,MTT均显著增高(均P<0.05);与模型组比较,治疗组各时间点亚组的rCBF、rCBV均显著降低,MTT均显著增高(均P<0.05)。结论脑出血后血肿周围组织存在低灌注状态,抵挡丸能显著改善脑出血后血肿周围组织血流灌注,减轻其病理损害,促进神经功能恢复。Objective To investigate the effects of the traditional Chinese medicine （Didang-pills） of activating blood circulation to dissipate blood stasis on neurological function,morphous and perfusion of brain tissue in intracerebral hemorrhage rats. Methods Fifty male Wistar rats were randomly divided into normal control group （n = 5） ,sham operation group （n = 5） ,intracerebral hemorrhage model group （model group,n = 20） and Didang- pills therapy group （therapy group,n = 20） . The last two groups were separately divided into 12 h,24 h,72 h,7 d 4 sub-groups. Intracerebral hemorrhage rat models were made by injection of autologous blood into the caudate nucleus. Rats in therapy group were givern 0. 08 g /L Didang-pills 3 ml by gastrointestinal perfusion twice a day. The regional cerebral blood flow （rCBF） ,regional cerebral blood volume （rCBV） and contrast agent mean transit time （MTT） at surrounding tissue of hematoma were measured by CT prefusion. The Bederson scores and pathological features were detected meanwhile. Results The Bederson scores of model group and therapy group were significantly higher than that of normal control group and sham operation group （all P 0. 05） ; and at 7 d after operation,the Bederson score of therapy group was significantly lower than that of model group （P 0. 05） . The pathological lesions of therapy group at all time points were significantly lighter than that of model group （all P 0. 05） . Compared with normal control group and sham operation group,the rCBF,rCBV were obviously decreased,and the MTT were obviously increased at model group and therapy group （all P 0. 05） ; while compared with model group,the rCBF, rCBV were obviously decreased,and the MTT was obviously increased at therapy group （all P 0. 05） . Conclusions The perfusion at surrounding tissue of hematoma is very low after intracerebral hemorrhage. The Didang-pills can significantly improve the perfusion at surrounding tissue of hematoma after intracerebral hemorrhage,a

关 键 词：抵挡丸 脑出血 脑血流 灌注 

分 类 号：R285.5[医药卫生—中药学]