HIV感染者CD56^(dim)CD16^+NK细胞表面活化受体和抑制受体的变化  

The expressions of activating receptor and inhibitory natural killer cell receptor(iNKR) on CD56^(dim)CD16^+ natural killer cells in HIV-infected individuals

作　　者：姜拥军[1] 陈欢[2] 何磊[1] 张子宁[1] 孙晓娟[1] 刘静[1] 韩晓旭[1] 丁海波[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室,沈阳110001 [2]吉林大学中日联谊医院检验科,长春130033

出　　处：《免疫学杂志》2010年第11期962-966,共5页Immunological Journal

基　　金：国家科技重大专项课题资助项目(2008ZX10001-001);国家重点基础研究发展计划(973计划()2006CB504206);辽宁省教育厅高等学校创新团队项目(辽教发[2008]117号2008T202)

摘　　要：目的探讨中国HIV感染者、AIDS病人CD56dimCD16+NK细胞表面活化受体NKG2D、抑制受体NKG2A的表达情况。方法应用流式细胞仪分析HIV感染者、AIDS病人外周血CD56dimCD16+NK细胞表面活化受体NKG2D、抑制受体NKG2A的表达。结果 CD56dimCD16+NK细胞表面,NKG2D+NKG2A-的NK细胞水平在HIV组、AIDS组明显低于正常对照组(P<0.01);AIDS组显著低于HIV组(P<0.05);HAART治疗组明显高于AIDS组(P<0.01)。NKG2A+NKG2D-的NK细胞水平在HIV组、AIDS组都明显高于正常对照组,AIDS组显著高于HIV组(P<0.01);HAART治疗组明显低于AIDS组(P<0.01),与正常对照组、HIV组之间无显著差异。HIV感染者、AIDS病人NKG2A+NKG2D-的NK细胞水平与CD4+T细胞负相关(r=-0.42,P<0.05),与病毒载量正相关(r=0.37,P<0.05)。结论 HIV感染使机体NKG2D+NKG2A-NK细胞水平下降,NKG2A+NKG2D-NK细胞水平升高,导致NK功能受损,抗病毒治疗可逆转相应的细胞水平。同时检测NK细胞表面活化受体和抑制受体的表达有助于艾滋病疾病进程的研究。To investigate the expressions of inhibitory receptor NKG2A and activating receptor NKG2D on CD56dim CD16＋NK cells in HIV/AIDS patients,we applied multi-colors flow cytometry analysis to study the expressions of inhibitory receptor NKG2A and activating receptor NKG2D on CD56dimCD16＋NK cells of peripheral blood of HIV/AIDS patients.The percentage of NKG2D＋NKG2A-was lower in HIV group and AIDS group than in healthy controls（P 0.01）,was lower in AIDS group than in HIV group（P 0.05）,and was higher in HAART group than in AIDS group（P 0.01）.The expression of NKG2A＋ NKG2D-NK was higher in HIV and AIDS groups than in healthy controls,was higher in AIDS group than in HIV group（P 0.01）,and was significant lower in HAART group than in AIDS group（P 0.01）,but showed no significant in healthy controls,HAART group,and HIV group.The expression of NKG2A＋NKG2D-on CD56dimCD16＋ NK cells in HIV/AIDS patients had negative correlation with absolute count CD4（r =-0.42,P 0.05） but positive correlation with PVL（r = 0.37,P 0.05）.The level of NKG2D＋NKG2A-NK cells declined,and the level of NKG2A＋ NKG2D-NK cells increased at the same time after HIV infection,which resulted in the impairment of NK function.The level of corresponding cells was reversed after anti-viral treatment.These data might provide insight into study of disease progression of AIDS by detecting activated receptor and inhibitory receptor simultaneously.

关 键 词：自然杀伤细胞 NKG2D NKG2A 人类免疫缺陷病毒 获得性免疫缺陷综合征 高效抗逆转录病毒治疗 

分 类 号：R512.91[医药卫生—内科学]