枸杞多糖对大鼠坐骨神经离断后创伤性神经瘤形成及其疼痛影响  

EFFECTS OF LYCIUM BARBARUM POLYSACCHARIDE ON FORMATION OF TRAUMATIC NEUROMA AND PAIN AFTER TRANSECTION OF SCIATIC NERVE IN RATS

作　　者：范洪伟[1] 邓春荣[2] 付健[1] 丁磊[3] 殷钢[3] 马玉林[3] 

机构地区：[1]宁夏医科大学临床医学院骨三科,银川750004 [2]鹤壁煤业集团总医院重症监护室 [3]宁夏医科大学第二附属医院骨科

出　　处：《中国修复重建外科杂志》2010年第11期1298-1301,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：宁夏回族自治区科技攻关项目(2009050)~~

摘　　要：目的探讨枸杞多糖(lycium barbarum polysaccharide,LBP)对大鼠坐骨神经离断后创伤性神经瘤形成及其疼痛的影响。方法健康SD大鼠40只,雌雄各半,体重200～220g,随机分为LBP组与对照组(n=20)。实验动物制备右侧坐骨神经离断(2cm)模型,术后1dLBP组腹腔注射LBP10mg/(kg·d),对照组腹腔注射等量生理盐水,共注射28d。术后观察趾甲及趾缺失情况,计数大鼠患肢自噬评分;术后28d,取坐骨神经离断近端0.5cm,光镜及透射电镜观察坐骨神经离断后创伤性神经瘤形成情况。结果术后4周,LBP组5只发生自噬现象,发生率25%;对照组12只发生自噬现象,发生率60%;两组比较差异有统计学意义(P<0.05)。光镜下见LBP组8只形成神经瘤,发生率40%;对照组16只形成神经瘤,发生率80%;差异有统计学意义(P<0.05)。光镜观察示对照组神经瘤内细胞杂乱生长,有肌纤维细胞侵润,部分神经瘤可见轴索及神经鞘细胞增生,形成小片状或条索状结构;LBP组神经纤维增生较少,神经纤维排列较整齐。透射电镜观察示对照组神经瘤部轴突分布多,纺锤状的成纤维细胞增多,可见较多胶原纤维,髓鞘增生溃变;而LBP组神经离断近端髓鞘较少,雪旺细胞及成纤维细胞较少,胶原纤维稀疏。结论 LBP对大鼠坐骨神经离断后自噬现象和创伤性神经瘤的形成有抑制作用。Objective To investigate the effects of lycium barbarum polysaccharide（LBP） on the formation of traumatic neuroma and pain after transection of sciatic nerve in rats. Methods Forty Sprague-Dawley（SD） rats,weighing 200-220 g,half male and half female,were allocated into 2 groups randomly：LBP group and control group（n=20 per group）.The right sciatic nerves were transected and 2 cm sciatic nerve were removed in all rats of the 2 groups.LBP were intraperitoneally injected in a volum of 10 mg/（kg·d） in the LBP group,while the same volum normal saline（NS） in the control group for 28 days.The deficiency of toenail and toe were observed to estimate the autophagy of the operated limb.Light microscope and transmission electron microscope were used to observe the formation of traumatic neuroma after transection of sciatic nerve. Results Autophagy was observed in 5 rats（25%） of LBP group and in 12 rats（60%） of control group at 4 weeks,showing significant difference（P 0.05）.Neuroma formed in 8 rats（40%） of LBP group and in 16 rats（80%） of control group,showing significant difference（P 0.05）.The observation of light microscope showed that there were unorganized growth cells in the neuroma,infiltrated muscle cells,the regeneration of axons and ensheathing cells to form small patch and funicular structure in the control group,while in the LBP group there were less proliferation of nerve fibers with a regular arrangement.Transmission electron microscope showed that there were lots of axons in nerve tumour,more fusoid fibroblasts,more collagen fiber,and hyperplasia and degenerated myelin sheath in the control group,while in the LBP group there were less myelin sheath in the proximal end of injuring nerves,less Schwann cells and fibroblasts,and sparsed collagen fibers.Conclusion LBP can inhibit autophagy and the formation of traumatic neuroma after transection of sciatic nerve in rats.

关 键 词：枸杞多糖 坐骨神经离断 疼痛 创伤性神经瘤 大鼠 

分 类 号：R285.5[医药卫生—中药学]