药物干预对哮喘小鼠模型Th_1/Th_2细胞因子表达的影响  

Interfering effects of thymosine and dexamethasone on TH_1/TH_2 cytokines in the asthma mouse model

作　　者：王岷[1,2] 陈文靖[1] 杨琳红[1] 刘磊[1] 吴大玮[2] 刘俊丽[1] 

机构地区：[1]山东中医药大学第二附属医院呼吸科,济南250001 [2]山东大学齐鲁医院ICU科,济南250012

出　　处：《山东大学学报（医学版）》2010年第10期48-50,共3页Journal of Shandong University:Health Sciences

摘　　要：目的探讨胸腺素-α和地塞米松对辅助型T淋巴细胞1型/辅助型T淋巴细胞2型(Th1/Th2)细胞因子在哮喘发病过程中的干预作用及其临床应用价值。方法将小鼠分为对照组、哮喘模型组、胸腺素干预组、地塞米松干预组。采用酶联免疫吸附试验(ELISA)法,测定支气管肺泡灌洗液(BALF)中白细胞介素-4(IL-4)、白细胞介素-5(IL-5)和干扰素-γ(IFN-γ)的水平。结果与对照组相比,哮喘组的嗜酸性粒细胞(EOS)、淋巴细胞、IL-4、IL-5升高,IFN-γ下降(P<0.05)。与哮喘组相比,胸腺素组和地塞米松组的EOS、IL-4、IL-5减少,胸腺素组IFN-γ增加(P<0.05)。对照组与哮喘组、哮喘组与胸腺素组、哮喘组与地塞米松组之间的IL-4/IFN-γ相比,差异均有统计学意义(P均<0.05)。结论 Th2优势反应在哮喘的发病机制中起重要作用,胸腺素-α可明显提高IFN-γ、Th1的水平,地塞米松可以降低IL-4、IL-5及Th2水平。Objective To explore the role of Th1/Th2 cytokines in the pathogenic process of asthma,interfering effects of thymosine-α and dexamethasone on Th1/Th2 and their clinical values.Methods An asthma model was set up and thymosine-α and dexamethasone were given.The rats were divided into the normal control group,the asthmic group,the thymosin group and the dexamethasone group.Levels of IL-4,IL-5 and IFN-γin bronchoalveolar lavage fluid（BALF） were detected by ELISA.Results There was a significant increase in the numbers of EOS and lymphocytes and levels of IL-4 and IL-5,while there was a significant decrease in the level of IFN-γ in the asthmic group compared with the normal control group（P0.05）.Number of EOS and levels of IL-4 and IL-5 decreased in the thymosine dexamethasone groups compared with the asthmic group,and the difference was significant（P0.05）.The level of IFN-γ in the thymosin group increased compared with the asthmic group and the difference was significant（P0.05）.For IL-4/IFN-γ,there were notable differences between the normal group and the asthmic group,between the asthmic group and the thymosin group,and between the asthmic group and the dexamethasone group（P0.05）.Conclusion The Th2 dominant reaction play an important role in the pathogenesis of asthma.Thymosin-α can notably increase the level of IFN-γ.Dexamethasone can lower the levels of IL-4 and IL-5.

关 键 词：T淋巴细胞 辅助诱导 胸腺素 地塞米松 小鼠 

分 类 号：R562.25[医药卫生—呼吸系统]