DMSO、地塞米松对于HBV感染HepCHLine-4细胞能力的影响  

Influences of DMSO and dexamethasone on HBV infection in HepCHLine-4 cells

作　　者：关彦彦[1] 姚永远[1] 王刚[1] 邵丽华[2] 赛林涛[1] 马立宪[1] 

机构地区：[1]山东大学齐鲁医院感染病科,济南250012 [2]山东大学公共卫生学院教学实验室,济南250012

出　　处：《山东大学学报（医学版）》2010年第10期65-68,84,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(81071340);山东省自然科学基金资助项目(Y2008C68)

摘　　要：目的评价二甲基亚砜(DMSO)、地塞米松对于乙型肝炎病毒(HBV)感染HepCHLine-4细胞能力的影响。方法 HBV感染前将HepCHLine-4细胞分为DMSO处理组(A组)、地塞米松处理组(B组)、DMSO及地塞米松处理组(C组)和对照组(D组)。含胎牛血清100mL/L的细胞培养基中,A组添加20mL/L DMSO,B组添加5×10-5mol/L地塞米松,C组添加20mL/L DMSO及5×10-5mol/L地塞米松,D组不添加上述两种试剂。用相应培养基培养各组细胞4d以备病毒感染。将HBV病毒颗粒加入各组细胞中于37℃中孵育24h。电化学发光法检测感染后各组细胞培养上清中HBsAg和HBeAg的滴度;荧光定量PCR检测感染后各组细胞培养上清的HBV DNA。结果 DMSO处理组HBsAg和HBeAg的滴度相对较高;地塞米松处理组HBV DNA值相对较高;DMSO及地塞米松处理组HBsAg和HBeAg的滴度及HBV DNA值均较高,其最高值分别是125.790IU/mL,4.784S/Co,5.930×105cop-ies/mL;对照组HBsAg和HBeAg的滴度及HBV DNA值均较低,其最高值分别是85.490IU/mL,1.896S/Co,3.729×104copies/mL。结论 DMSO、地塞米松有提高HepCHLine-4细胞被HBV自然感染能力的趋势。Objective To evaluate the influences of dimethyl sulfoxide（DMSO） and dexamethasone on the ability of HepCHLine-4 cells to Hepatitis B virus（HBV） infection.Methods HepCHLine-4 cells were divided into four groups： the DMSO group,in which HepCHLine-4 cells were cultured in Dulbecco′s modified Eagle′s medium（DMEM） with 20 mL/L DMSO for 4 days;the dexamethasone group,in which HepCHLine-4 cells were cultured in DMEM with 5×10-5mol/L dexamethasone for 4 days;the DMSO plus dexamethasone group,in which HepCHLine-4 cells were cultured in DMEM with 20 mL/L DMSO and 5×10-5mol/L dexamethasone for 4 days;and the control group,in which HepCHLine-4 cells were cultured in DMEM without DMSO or dexamethasone for 4 days.Further,HepCHLine-4 cells were incubated with HBV at 37 ℃ for 24 hours.Hepatitis B surface antigen（HBsAg） and hepatitis Be antigen（HBeAg） in the supernatant of infected cells were detected by electrochemiluminescence（ECL） and HBV DNA was detected by fluorescence quantitative real-time polymerase chain reaction（FQ-RCR）.Results 1） In the DMSO group the titer of HBsAg and HBeAg was relatively high;2） In the dexamethasone group the titer of HBV DNA was relatively high;3） In the DMSO plus dexamethasone group the titer of HbsAg,HBeAg and HBV DNA were higher.The highest levels were 125.790 IU/mL,4.784S/Co and 5.930 ×105 copies/mL respectively;4） In the control group the titer of HbsAg,HBeAg and HBV DNA were lower,with the highest levels being 85.490 IU/mL,1.896S/Co,3.729×104 copies/mL respectively.Conclusion DMSO and dexamethasone can improve the ability of HepCHLine-4 to HBV infection.

关 键 词：乙肝病毒 二甲基亚砜 地塞米松 感染 

分 类 号：R512.6[医药卫生—内科学]