益气补肾中药脑神康胶囊的质量控制标准研究  

Quality control of invigorating Qi and tonifying kidney "NaoShenKang" Capsule

作　　者：钟丽红[1] 赵宇[2] 刘德山[3] 

机构地区：[1]山东大学齐鲁医院药剂科,济南250012 [2]山东大学药学院生药研究所,济南250012 [3]山东大学齐鲁医院中医科,济南250012

出　　处：《山东大学学报（医学版）》2010年第10期116-119,共4页Journal of Shandong University:Health Sciences

基　　金：山东省中医药管理局科学基金资助项目(2007-133)

摘　　要：目的建立脑神康胶囊多糖及主要活性成分黄芪甲苷、葛根素、淫羊藿苷、小檗碱以及熟地黄的限量成分5-羟甲基糠醛(5-HM F)的含量测定方法。方法采用硫酸-苯酚法测定脑神康中多糖的含量;采用高效液相蒸发光散射检测法(HPLC-ELSD)测定黄芪甲苷的含量;采用高效液相色谱法(HPLC)测定葛根素、淫羊藿苷、小檗碱和5-羟甲基糠醛的含量。结果脑神康胶囊中多糖、黄芪甲苷、葛根素、淫羊藿苷、小檗碱含量分别为5.01%、0.007 96%、0.434%、0.061 8%、0.108%;限量成分5-羟甲基糠醛含量为0.021 9%。结论初步建立了益气补肾中药脑神康胶囊的质量控制方法,为脑神康胶囊的进一步开发研究奠定了基础。Objective To establish methods to determine polysaccharides,Astragaloside IV,kakkonein,icariin,berberinum and 5-HMF in the ＂Naoshenkang＂ Capsule.Methods The content of polysaccharides in the＂Naoshenkang＂ Capsule was determined by sulfuric acid-phenol method.The content of Astragaloside IV was determined by HPLC-ELSD,and other compounds,including kakkonein,icariin,berberinum and 5-HMF,in the capsule were determined by HPLC-UV/Vis.Results The contents of polysaccharides,Astragaloside IV,kakkonein,icariin,berberinum and 5-HMF in the ＂Naoshenkang＂ Capsule were 5.01%、 0.007 96%、 0.434%、0.061 8%、 0.108% and 0.021 9%,respectively.Conclusion The preliminary quality control method is established for the＂Naoshenkang＂ Capsule.The result from the assays provided useful data for the further research of the＂Naoshenkang＂ Capsule.

关 键 词：脑神康 多糖 黄芪甲苷 活性成分 5-羟甲基糠醛 色谱法 高效液相 

分 类 号：R944.5[医药卫生—药剂学] R917[医药卫生—药学]