小半夏汤防治大鼠化疗性异食癖的5-HT机制研究  

5-HT mechanism of Xiaobanxiatang(小半夏汤) in preventing chemotherapy induced pica in rats

作　　者：王丽霞[1] 王静[1] 聂克[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中药药理与临床》2010年第5期7-9,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：教育部留学回国人员科研启动基金(教外司留[2007]1108号);山东省高等学校科技计划项目(编号:J09LF07)

摘　　要：目的:观察小半夏汤防治大鼠化疗性异食癖的5-HT作用机制。方法:腹腔注射顺铂6mg/kg建立大鼠异食癖模型,分别灌胃小半夏汤水提液和醇提液1.6g/kg,连续3d,阳性药对照组灌胃昂丹司琼1.2mg/kg。每天记录大鼠高岭土摄入量。RP-HPLC法测定大鼠血清及脑组织5-HT和5-H IAA水平,免疫组化法观察脑及回肠5-HT与5-HT3A受体阳性细胞表达。结果:小半夏汤水提液和醇提液均可抑制化疗性大鼠异食癖,醇提液的抑制作用强于水提液;均可降低血清及脑组织5-HT、5-H IAA水平;均可减少脑及回肠5-HT3A受体阳性细胞表达,减少回肠5-HT表达。结论:小半夏汤防治化疗性恶心呕吐作用与抑制小肠5-HT释放,下调小肠和脑5-HT3受体有关。Objective： To investigate the 5-HT mechanism of Xiaobanxiatang（XBXT） in preventing chemotherapy-induced pica in rats.Methods： Wistar male rats were injected intraperitoneally with cisplatin 6mg/kg to induce pica.The aqueous or ethanol extraction of XBXT 1.6g/kg was administered by gavage for 3d respectively.Kaolin consumption was recorded everyday in the following 72h.The content of 5-HT and 5-HIAA in serum and brain were determined in RP-HPLC.5-HT and 5-HT3A receptor expression of ileum and brain were observed by immunohistochemistry.Results： Both aqueous and ethanol extraction of XBXT could significantly inhibit cisplatin induced pica in rats,lower the content of 5-HT and 5-HIAA in serum and brain,reduce the expression of 5-HT3A receptor in ileum and brain,reduce the expression of 5-HT in ileum.Conclusion： The present studies indicated that XBXT can inhibit chemotherapy induced nausea and vomiting,inhibiting 5-HT release and down-regulating 5-HT3 receptor might be part of its mechanism.

关 键 词：小半夏汤 止呕 顺铂 异食癖 5-HT 

分 类 号：R285.5[医药卫生—中药学]