复方丹参多元释药微丸对心肌细胞缺氧/复氧损伤的保护作用研究  

Protection of compound danshen multi-release pellets on hypoxia/reoxygenation induced injury in myocardial cells

作　　者：汤喜兰[1] 徐国良[1] 单义民[1] 张启云[1] 李冰涛[1] 唐剑彬[1] 郑琴[1] 杨明[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中药药理与临床》2010年第5期15-17,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家十一五支撑计划资助项目(No.2006BAI09B08-01);江西省卫生厅中医科研计划(No.2009A059;2009A061)

摘　　要：目的:运用血清药理学的方法,探讨复方丹参多元释药微丸对心肌细胞缺氧/复氧损伤的保护作用,评价复方丹参多元释药微丸制剂的有效性。方法:单次灌胃给予市售复方丹参片和复方丹参多元释药微丸(折算生药材2911.14 mg/kg),于给药后0.25,0.5,0.75,1,2,4,8,12 h眼眶取血制备含药血清,并将不同时间点的含药血清作用于心肌细胞,检测心肌细胞的活性。结果:与模型组比较,市售复方丹参片4,8 h的含药血清对缺氧/复氧损伤心肌细胞有促增殖作用,而复方丹参多元释药微丸0.25,0.5,0.75,1,4,8,12 h的含药血清均能够显著促进缺氧/复氧损伤心肌细胞的增殖。结论:复方丹参多元释药微丸对心肌细胞缺氧/复氧损伤具有良好的保护作用,相对于复方丹参片,具有起效快,作用时间长的特点。Objective： To investigate the protective effects of compound danshen multi-release pellets（CDMRP） on hypoxia/reoxygenation（H/R） induced injury in myocardial cells by serum pharmacological method.Methods： Sprague-Dawley rats had been administrated compound danshen tablet（CDT） and compound danshen multi-release pellets with the content of crude drugs 2911.14 mg/kg.The medicated serum of CDT and CDMRP were prepared at the time of 0.25,0.5,0.75,1,2,4,8,12h after single oral administration and added to myocardial cells.The activity of myocardial cells were detected.Results： Compared with H/R model group,The medicated serum of CDT 4,8 h and CDMRP 0.25,0.5,0.75,1,4,8,12 h had apparent protective effects against proliferation inhibition caused by hypoxia/reoxygenation induced injury in myocardial cells.Conclusions： CDMRP has good protective effects on hypoxia/reoxygenation induced injury in myocardial cells with shorter response time and longer duration than CDT.

关 键 词：复方丹参多元释药微丸 心肌细胞 缺氧/复氧损伤 

分 类 号：R285.5[医药卫生—中药学]