纳米雄黄对耐药性白血病细胞的凋亡诱导作用  

The apoptosis of multi-drug resistant leukemia cells induced by realgar nanoparticle

作　　者：王永胜[1] 易娟 陈静[1] 魏虎来[1] 李红玲[2] 

机构地区：[1]兰州大学基础医学院,甘肃省新药临床前研究重点实验室,兰州730000 [2]甘肃省人民医院,兰州730000

出　　处：《中药药理与临床》2010年第5期33-36,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：甘肃省中医药科学技术研究课题(GZK-2009-16、GZK-2010-17)

摘　　要：目的:研究纳米雄黄对耐药性白血病K562/ADM细胞的凋亡诱导作用。方法:采用机械研磨法制备纳米雄黄。以白血病多药耐药K562/ADM细胞株为靶细胞,采用MTT法检测细胞的增殖活性,AnnexinV/PI双染色法检测细胞凋亡,流式细胞术检测BAX、Bcl-2、P53蛋白的表达水平以及Caspase-3活性。结果:纳米雄黄显著抑制K562/ADM细胞增殖。20μg/ml和50μg/ml纳米雄黄处理48h,K562/ADM细胞凋亡率显著增高,分别为13.35%和72.70%;P53蛋白表达由对照的(33.25±0.75)%增高到(75.55±2.05)%和(87.25±2.15)%;BAX蛋白由对照的(72.05±0.75)%增高到(99.05±0.35)%和(99.80±0.01)%,Bcl-2蛋白表达轻度增高,BAX/Bcl-2比值增高。20μg/ml和50μg/ml纳米雄黄处理24h,Caspase-3活性显著增强。结论:纳米雄黄可诱导白血病多药耐药K562/ADM细胞发生凋亡。Objective： To explore apoptosis-inducing effects of the realgar nanoparticle（nano-realgar） on multi-drug resistant leukemia K562/ADM cells.Methods： The nano-realgar was prepared via mechanical milling using a high-energy planetary ball mill.MTT assay was used to detect the proliferating activity of K562/ADM cells,and the cellular apoptosis was assed with Annexin V/PI double staining.Flow cytometry（FCM） technique was employed to assess intracellular BAX,Bcl-2 and P-53 protein expression and the activity of Caspase-3.Results： Nano-realgar significantly inhibited the proliferation of K562/ADM cells.K562/ADM cells were treated with 20 g/ml and 50 g/ml of nano-realgar for 48h,The apoptotic rate of the cells detected with Annexin V/PI staining was 13.35% and 72.70%,respectively.The P53 expression of protein in target cells obviously increased,from（33.25±0.75）% to（75.55±2.05）% and（87.25±2.15）%.The expression of BAX protein increased from（72.05±0.75）% to（99.05±0.35）% and（99.80±0.01）%,and the Bcl-2 protein displayed a slightly increase,the ratio of BAX and Bcl-2 markedly rise.After treatment with 20 g / ml and 50 g/ml nano-realgar for 24h to 48h,Caspase-3 activity of K562/ADM cells significantly enhanced.Conclusion： Nano-Realgar significantly induces the apoptosis of multidrug resistance leukemia K562/ADM cells.

关 键 词：纳米雄黄 多药耐药 细胞凋亡 白血病 

分 类 号：R285.5[医药卫生—中药学]