去乙酰紫玉盘素抑制HepG2细胞增殖和诱导凋亡分化作用  

Effect of desacetyluvaricin on proliferation,apoptosis and differentiation of human hepatocarcinoma cell line HepG2 in vitro

作　　者：任凤梅[1] 舒秉俊[1] 罗时辉[1] 王鹰[1] 章跃平[1] 徐安[1] 

机构地区：[1]南昌市第二医院,南昌330003

出　　处：《中药药理与临床》2010年第5期36-39,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：江西省卫生厅中医药科研基金课题(课题编号:2009A075)

摘　　要：目的:探讨番荔枝内酯单体去乙酰紫玉盘素(Dsacetyluvaricin)体外抑制人肝癌细胞株HepG2增殖,诱导凋亡和分化的作用。方法:将不同浓度的Dsacetyluvaricin与HepG2细胞在体外常规培养,应用MTT比色法、流式细胞技术、透射电子显微镜、荧光显微镜检测其对细胞增殖的抑制作用、DNA含量、细胞周期各时相及细胞超微结构的变化。结果:Dsacetyluvaricin对体外培养的人肝癌HepG2细胞的生长增殖具有明显的抑制作用,抑制率与剂量和作用时间呈显著正相关关系;能阻滞细胞增殖于G0-G1期,使S期细胞减少,细胞凋亡率增加,核质比例下降,细胞出现典型的形态学改变。结论:Dsacetyluvaricin对HepG2细胞存在时间和剂量依赖性增殖抑制作用,可诱导细胞的凋亡,提高细胞的分化程度,是很有潜力的抗肝癌药物。Objective： To investigate the effect of desacetyluvaricin on proliferation,apoptosis and differentiation of human hepatocarcinoma cell line HepG2 in vitro.Methods： Different concentrations of desacetyluvaricin and the different time of cultivation were used to treat HepG2 cells.Proliferation inhibitory rate was evaluated by MTT assay,DNA content,cell cycle and super-microstructure were observed through flow cytometry,electronic microscope and fluorescence microscope.Results ：Desacetyluvaricin could inhibit HepG2 cell proliferation viability within a certain range of treating time and does.A majority of HepG2 cells were arrested in G0-G1 phase and a progressive decline of cells was seen in S phase.The apoptosis rate was increased and nucleo-cytoplasmic ratio was decreased of HepG2 cells.The HepG2 cells apoptosis was confirmed by cell typical cell morphology.Conclusion： Desacetyluvaricin have a time and does-dependent growth inhibition effect and can induce apoptosis,increase the differentiation degree of HepG2 cells.

关 键 词：番荔枝 dsacetyluvaricin 人肝癌细胞株 细胞凋亡 细胞分化 

分 类 号：R285.5[医药卫生—中药学]