丙型肝炎肝组织中HCV RNA和HCV NS5抗原表达及意义  

Expression and significance of HCV RNA and HCV NS5 antigen in liver tissues of patients with hepatitis C

作　　者：赵西平[1] 沈汉馨[1] 田德英[1] 张东绅[1] 彭志海[1] 杨东亮[1] 郝连杰[1] 

机构地区：[1]同济医科大学附属同济医院

出　　处：《世界华人消化杂志》1999年第6期516-518,共3页World Chinese Journal of Digestology

摘　　要：目的研究丙型肝炎肝组织中ＨＣＶＲＮＡ与ＨＣＶＮＳ５抗原分布及其与肝组织损伤的关系．方法应用原位杂交和免疫组化方法检测３４例丙型肝炎病毒（ＨＣＶ）感染者肝组织中ＨＣＶＲＮＡ和ＨＣＶＮＳ５抗原表达状况，采用Ｋｎｏｄｅｌ方法评价肝组织病理损伤程度．结果分别有２１例（６１８％）和２４例（７０６％）患者肝组织中检出ＨＣＶＲＮＡ和ＨＣＶＮＳ５抗原．ＨＣＶＲＮＡ阳性信号主要位于肝细胞浆中，在胆管上皮细胞和炎性浸润细胞中也可检出，其分布与肝组织炎症坏死无固定解剖学关系．ＨＣＶＲＮＡ与肝组织中ＨＣＶＮＳ５抗原表达正相关，但二者在肝组织中的分布存在一定差异．多因素分析显示ＨＣＶＮＳ５抗原的表达与肝组织损伤相关．结论丙型肝炎肝组织中ＨＣＶＲＮＡ和ＨＣＶＮＳ５抗原表达相关。AIM To investigate the relationship of HCV RNA and HCV NS5 antigen expression and their pathological significance in patients with hepatitis C. METHODS HCV RNA and HCV NS5 antigen in liver tissues of 34 patients with hepatitis C were detected using in situ hybridization and immunohistochemical methods, respectively. Liver pathological damage was evaluated with Knodell's histology activity index (HAI). RESULTS HCV RNA was detected in 21 (61 8%) cases, while HCV NS5 antigen was in 24 (70 6%) cases. The positive signals of HCV RNA were mainly located in cytoplasm of hepatocytes. HCV RNA could be detected in bile duct epithelial cells as well as infiltrated inflammatory cells in a few cases. There was no obvious topography link of HCV RNA positive cells with liver necroinflammation. The expression of HCV RNA and HCV NS5 antigen in liver had a positive correlation( r =0 543, P <0 01),but with some difference in their distribution. Multivariate analysis showed that the expression level of HCV NS5 antigen was obviously correlated with liver injury. CONCLUSION The expression of HCV RNA in liver is correlated with HCV NS5 antigen, and the liver injury of chronic hepatitis C is related to the activity of HCV in liver.

关 键 词：丙型肝炎 RNA 抗原 HCV 肝组织损伤 

分 类 号：R512.630.2[医药卫生—内科学]