兔胆道口括约肌细胞的培养及结构形态学观察  

Culture and morphologic observation of rabbit Oddi's sphincter cells

作　　者：张劲松[1] 魏经国 吴军正[1] 陈建元 

机构地区：[1]第四军医大学

出　　处：《世界华人消化杂志》1999年第4期316-319,共4页World Chinese Journal of Digestology

摘　　要：目的通过胆道口括约肌（ｓｐｈｉｎｃｔｅｒｏｆＯｄｄｉ，ＳＯ）细胞培养及形态学观察，为胆道口括约肌的功能结构研究提供新的途径．方法通过对纯种兔ＳＯ的解剖基础研究，采用原代细胞培养技术（复合酶消化法和组织块培养法）进行体外ＳＯ细胞培养，免疫细胞化学和电镜鉴定，并重点对细胞骨架进行了观察．结果利用兔ＳＯ“壁外型”的特点，成功进行了ＳＯ平滑肌细胞的培养，其纯度＞９０％，细胞骨架具有平滑肌细胞的典型特征．结论兔胆道口括约肌可以进行纯度较高的培养，从而为进一步的ＳＯ相关研究提供了新方法．AIM To study the dyskinesia of Oddi’s sphincter in rabbits. METHODS Through topographic study of pure bred rabbit sphincter of Oddi (SO), and primary culture methods including tissue block and combined enzyme digest method, SO cells were cultured, and identified by immunocytochemistry and electron micrography. RESULTS SO cells were successfully cultured on the basis of SO “extra duodenal” characteristics, with a purity of more than 90%. Cellular skeleton had the characteristics of smooth muscle cells. CONCLUSION Culture of SO cells may be a new way to study the structure and function of SO.

关 键 词：胆道括约肌 细胞培养 细胞骨架 

分 类 号：R329.4[医药卫生—人体解剖和组织胚胎学] R329-33[医药卫生—基础医学]