大鼠睾丸扭转复位及减压治疗对睾丸的影响  

A preliminary study of the effects of testicular torsion/detorsion and decompression on testicular tissue in rats

作　　者：潘明[1] 马峥[2] 朱英坚[2] 刘国华[1,2] 

机构地区：[1]上海交通大学附属第一人民医院儿外科,上海200080 [2]上海交通大学附属第一人民医院泌尿外科,上海200080

出　　处：《临床儿科杂志》2010年第11期1069-1072,共4页Journal of Clinical Pediatrics

摘　　要：目的研究睾丸扭转复位及减压治疗对睾丸的影响,为睾丸扭转的预后判断、治疗方法的选择等提供新的理论依据。方法将30只SD雄性大鼠随机分成5组,制成睾丸扭转复位及复位+减压治疗的模型。分别设立空白对照组及实验A～D组,睾丸扭转/复位组(A组)、睾丸扭转/复位+减压治疗组(B组)喂养至术后1 d处死;睾丸扭转/复位组(C组)、睾丸扭转/复位+减压治疗组(D组)喂养至术后1个月处死。应用化学检测和组织学分析方法,观察睾丸大体标本的变化、睾丸组织内丙二醛(MDA)含量的变化及睾丸组织Johnsen's评分。结果五组睾丸MDA分别为3.18±0.22(空白对照组)、9.54±2.05(A组)、7.92±1.38(B组)、6.67±0.61(C组)、4.30±1.81(D组),实验组术侧睾丸的MDA含量显著高于自身对侧(P<0.05)。D组MDA含量比C组明显下降(P<0.05)。单纯复位组的术后睾丸标本比自身对侧和减压治疗组有明显萎缩;五组Johnsen's评分分别为10±0、7.2±0.18、8.2±0.19、2.2±0.19、9.2±0.18。D组比C组明显提高(P<0.05)。结论睾丸扭转复位+减压治疗能明显减少扭转侧睾丸生殖细胞凋亡,减轻脂质过氧化程度,可能有利于睾丸组织结构与功能的恢复。Objective To investigate the effect of treatment of testicular torsion/detorsion and decompression（＂Testicular fasciotomy＂ combined with a tunica vaginalis patch） on testis tissue.To provide a new theoretical basis for the prognosis and the treatment of testicular torsion.Methods Thirty Sprague-Dawley rats were randomly divided into 5 groups,used as testicular torsion/detorsion and decompression models.In the control group（sham-operation control）,1 day experimental group： group A（torsion/detorsion） and group B（torsion/detorsion plus decompression）.1 month experimental group： group C（torsion/detorsion） and group D（torsion/detorsion plus decompression）.Testicular tissue was performed to observe morphological changes,Malondialdehyde（MDA） level were measured and Johnsen＇s score were evaluated.Results MDA content in the torsion testes were 3.18 ± 0.22、 9.54 ± 2.05、 7.92 ± 1.38、 6.67 ± 0.61、 4.30 ± 1.81 in group control、 A、 B、 C and D.MDA content in testis of the operated side were significantly higher than that of the contralateral side and that of the control group（P 0.05）,significantly lower in group D than in C（P 0.05）.Significant differences were observed in Johnsen＇s score among A（7.2 ± 0.18）,B（8.2 ± 0.19）,C（2.2 ± 0.19）,D（9.2 ± 0.18）,significantly higher in group D than that in C（P 0.05）.Conclusions Treatment of testicular decompression could significantly reduce the apoptosis of spermatogenic cells,decrease the level of lipid peroxidation and may improve testicular tissue structure and function.

关 键 词：睾丸扭转 减压治疗 丙二醛 Johnsen's评分 

分 类 号：R726.9[医药卫生—儿科]