慢性特发性荨麻疹患者嗜碱粒细胞转录激活蛋白-1的相关研究  

Transcription activator protein 1 （AP-1）-related study in basophils from patients with chronic idiopathic urticaria

作　　者：马一平[1] 姚煦[1] 林麟[1] 周武庆[1] 崔盘根[1] 

机构地区：[1]中国医学科学院北京协和医学院皮肤病研究所,南京210042

出　　处：《中华皮肤科杂志》2010年第11期762-765,共4页Chinese Journal of Dermatology

基　　金：中央级公益性科研院所基本科研业务专项基金

摘　　要：目的 探讨转录激活蛋白1（AP-1）在慢性特发性荨麻疹（CIU）发病中的作用.方法 采用嗜碱粒细胞免疫磁珠分离技术,提取CIU患者和正常人对照者外周血嗜碱粒细胞,进一步分离嗜碱粒细胞核蛋白.分别通过TransAM AP-1 Family试剂盒和Western印迹法,研究CIU患者和正常人对照者外周血嗜碱粒细胞AP-1家族因子DNA结合活性的改变及P-c-jun蛋白的表达情况.结果 CIU组与正常人对照组相比,嗜碱粒细胞AP-1家族因子DNA结合活性存在一定的差异.CIU组P-c-jun、c-fos、Fos-B、Jun-B、Jun-D因子DNA结合活性高于正常人对照组,其中P-c-jun、Jun-D差异有统计学意义（P〈0.05）.Fra-1因子DNA结合活性检测中,CIU组低于正常人对照组,但差异无统计学意义（P〉0.05）.Western印迹法发现,CIU组P-c-jun（Ser73）蛋白表达较之正常人对照组升高（P〈0.05）,而P-c-jun（Ser63）蛋白表达两组之间差异无统计学意义.结论 CIU患者嗜碱粒细胞AP-1家族部分因子DNA结合活性的改变及P-c-jun（Ser73）蛋白表达升高可能为CIU发病环节之一.Objective To investigate the role of AP-1 in the pathogenesis of chronic idiopathic urticaria （CIU）. Methods By using immunomagnetic separation technology, peripheral blood basophils were isolated from 10 CIU patients and 10 normal human controls followed by the extraction of nuclear protein from the basophils. TransAMTM AP-1 family kit was used to detect the DNA binding activity changes of AP-1 family transcription factors in basophils, and Western blotting to detect the expression of P-c-jun protein. Results There were some differences in the DNA binding activity of AP-1 family transcription factors in basophils between CIU patients and normal controls. The DNA binding activity of Phospho-c-jun, c-fos, Fos-B, Jun-B and Jun-D factors was increased in CIU patients compared with the controls, and the increase in that of P-c-jun and Jun-D was statistically significant （both P 〈 0.05）. There was an insignificant decrease in the DNA binding activity of Fra-1 factor in the CIU patients compared with the controls （P 〉 0.05）. The P-c-jun （Ser73） protein expression was higher in CIU patients than that in the controls （0.527 ± 0.312 vs. 0.435 ± 0.042, P 〈 0.05）,whereas there was no significant difference in the P-c-jun （Ser63） protein expression level. Conclusion Some changes in DNA binding activity of AP-1 and overexpression of P-c-jun （Ser73） protein in basophils may be involved in the pathogenesis of CIU.

关 键 词：荨麻疹 嗜碱粒细胞 转录激活因子1 

分 类 号：R758.24[医药卫生—皮肤病学与性病学]