高温和四环素对Wolbachia诱导孤雌产雌短管赤眼蜂的影响  

Effect of High Temperature and Tetracycline on Thelytokous Parthenogenesis Trichogramma pretiosum Riley Induced by Wolbachia

作　　者：潘雪红[1,2] 何余容[1] 

机构地区：[1]华南农业大学昆虫生态研究室,广州510642 [2]广西壮族自治区甘蔗研究所,南宁530007

出　　处：《华中农业大学学报》2010年第6期687-691,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30370966);广西农业科学院基金项目(2007006);广西甘蔗研究所基本科研业务专项(G2009009)资助

摘　　要：采用持续高温(30℃和33℃恒温)饲养生长24 h后的短管赤眼蜂和不同质量浓度四环素(四环素+30%的蜂蜜水配成)喂养成虫的方法,观察各处理对孤雌产雌短管赤眼蜂(Trichogramma pretiosumRiley)性别和产卵量的影响。结果表明:高温处理后,短管赤眼蜂由原来的完全孤雌产雌变成了完全孤雌产雄,并只出现少量雌雄嵌合体(数量不到1%),其产卵量也比未处理的显著降低,说明高温能将短管赤眼蜂体内的沃尔巴克氏体(Wolbachia)完全并快速杀死;用1、10、100 mg/mL 3个质量浓度的四环素对完全产雌孤雌生殖的短管赤眼蜂连续处理8代后,每代均出现少量的雄蜂和雌雄嵌合体,且随着处理代数的增加,短管赤眼蜂平均产卵量开始有下降的趋势,在F2代达到最低,随后又呈上升趋势,在F7和F8代平均产卵量甚至高于F1代,各处理代产卵量较不处理代F0均显著降低。The Trichogramma larvae were raised under constant high temperature（30 ℃ and 33 ℃） 24 hours after being hatched and their adults were fed with tetracycline of different concentrations（tetracycline＋30% honey water： 1,10,100 mg/mL）.The effects of the above treatment on the sex and egg production of the thelytokous parthenogenesis Trichogramma pretiosum were studied.The results showed that high temperature had a great effect on T.pretiosum,which would become arrhenotoky.It showed that high temperature can kill Wolbachia rapidly.Compared with the untreated T.pretiosum,T.pretiosum growing in high temperature had lower egg production and some intersexes（less than 1%） emerged.The effect of tetracycline on the sex and the egg production of T.pretiosum was also studied.Adults of completely thelytokous parthenogenesis T.pretiosum were continuously provided with tetracycline＋30% honey water.After treatment there were some males and intersexes appearing in each generation.Most of the progeny were still females.After continuous treatment of 8 generations with different tetracycline concentrations,the mean number of eggs laid by treated T.pretiosum adults had a decrease tendency at first,and reached the least at F2.And the fecundity tended to ascend later,and the mean number of eggs at F7 and F8 was even higher than that of F1.The mean number of eggs of each generation by tetracycline treatment was distinctly less than that of the untreated（F0）.

关 键 词：短管赤眼蜂 高温 四环素 沃尔巴克氏体 生殖力 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治] S477.1[农业科学—植物保护]