氰戊菊酯对草鱼肝微粒体CYP3A活性的影响  

Effect of Fenvalerat on Activtity of Hepatic CYP3A in Grass Carp

作　　者：陈秀荣[1,2] 罗宇良[1,2] 曾令兵[1] 闫海燕[2] 

机构地区：[1]中国水产科学研究院淡水生态与健康养殖重点开放实验室/中国水产科学研究院长江水产研究所,荆州434000 [2]华中农业大学水产学院,武汉430070

出　　处：《华中农业大学学报》2010年第6期768-771,共4页Journal of Huazhong Agricultural University

基　　金：国家"973"项目子课题(2009CB118706);中国水产科学研究院淡水生态与健康养殖重点开放实验室开放基金(2010FEA03011)资助

摘　　要：通过设计1.2、6.0、12.0μg/L 3个氰戊菊酯质量浓度组,1个空白对照组,1个恩诺沙星阳性对照组,进行氰戊菊酯对草鱼肝微粒体CYP3A活性影响及草鱼CYP3A活性的组织分布研究。结果表明:CYP3A存在于肝脏、鳃、肾脏、脾、肠、脑组织中,其中肝脏中活性最高;氰戊菊酯能显著抑制CYP3A的活性,随暴露时间的延长抑制作用增强,但抑制趋势减弱,3 d内趋势最强,各试验浓度组之间的差异不显著。建议临床用药,尤其是联合用药、多次用药时要充分考虑氰戊菊酯对代谢酶活性的影响。The CYP3A activity in different tissues of grass carp and the effect of fenvalerat on the hepatic CYP3A activity had been studied.There were three test concentrations,1.2,6.0,12.0 μg/L,respectively,a blank control,and a positive control of enrofloxacin to study the effect of fenvalerat on the hepatic CYP3A activtity in grass carp.The results showed that CYP3A activity was detected in liver,gill,kidney,spleen,intestine and brain,with the highest activity in liver.Fenvalerate significantly inhibited the hepatic CYP3A activity and this effect was enhanced with the exposure time.The effect was weaken after 3 days.There were no significant differences between the test groups.Therefore,the clinical use of this pyrethroid,especially combination therapy in aquaculture should be in caution.

关 键 词：草鱼 CYP3A活性 氰戊菊酯 药物代谢 

分 类 号：S948[农业科学—水产养殖]