上海某禽宰场30只鸭子中甲型流感病毒的检测  

Detection of Type A Influenza Virus in 30 Randomly Selected Health Ducks in a Shanghai Duck Sacrifice Ground

作　　者：陆嘉良[2] 周晓明[1,2] 潘浩 林玉尊[2] 居丽雯[2] 姜庆五[2] 顾惠心[2] 俞顺章[2] 

机构地区：[1]上海市公共卫生临床中心流行病学部,上海201508 [2]复旦大学公共卫生学院,上海200032 [3]江苏省卫生防疫站急性传染病科

出　　处：《公共卫生与临床医学》2010年第4期284-286,290,共4页Public health and dinical medicine

基　　金：上海市卫生局科学发展基金：国家自然科学基金青年基金30200233项目;复旦大学2002年度青年科学基金资助项目

摘　　要：目的了解近期禽宰场流感病毒在鸭子中的携带情况。方法在禽宰场随机选择正常健康状态待宰鸭子30只，分别取咽和肛门混合拭子，使用鸡胚培养扩增，血凝法初筛，甲型流感病毒NP蛋白保守核酸序列作扩增鉴定。结果在30份样品中，经鸡胚培养扩增，1：50的血凝法初筛50％为阳性，经核酸聚合酶链反应扩增鉴定10份样品中，9份为甲型流感病毒。结论用本方法在正常鸭子中甲型流感病毒的可检出携带率约为45％。Objective To determine the current type A influenza virus carry rate in health ducks in Shanghai poultry sacrifice ground; Methods The cotton swab samples from duck mouth and anal were collected from one of Shanghai duck sacrifice sites, both chicken blood agglutination test and PCR amplification of conservered type A influenza virus NP gene sequences were employed to identify type A influenza virus infection. Results 50% of 30 samples were positive in 1：50 blood agglutination test, 9 samples of 10 positive samples could be amplified with NP gene fragment. Conclutions After the large outbreak of type A influenza virus infection in 2009, approximate 45% of health ducks carry type A influenza viruses in Shanghai, which may pose a potential risk for new outbreak in the future.

关 键 词：甲型流感病毒 PCR反应 鸭子 血凝法 

分 类 号：R511.7[医药卫生—内科学] R446.61[医药卫生—临床医学]