改进酶稳定性的定向进化技术  

Directed Evolution for Improvement of Enzyme Stability

作　　者：程峰[1] 董俐住[1] 柳志强[1] 郑裕国[1] 

机构地区：[1]浙江工业大学生物工程研究所,杭州310014

出　　处：《生物技术通报》2010年第12期93-99,共7页Biotechnology Bulletin

基　　金：国家高技术研究发展计划(2008AA02Z247)

摘　　要：酶作为一种生物催化剂,以其独特的优良特性,在"绿色化学"和"清洁生产"中得到了广泛的应用。随着酶定向进化技术的建立和发展,通过定向进化改进酶稳定性的研究越来越多。详细综述了各种定向进化方法的特点及在提高酶稳定性方面的应用,并从结构和功能的角度进一步解释了相关机理。As a biological catalyst,enzymes with unique excellent properties,have been widely used in ＂green chemistry＂ and ＂cleaner production＂.With the emergence and development of directed evolution technology,there are a large number of studies on improvements of enzyme stability through directed evolution.This paper elaborated the characteristics of various methods of directed evolution and their application to improve enzyme stability,further explained the mechanism on the level of structure-function relationship.

关 键 词：酶的稳定性 定向进化 DNA改组 

分 类 号：Q814[生物学—生物工程]