非寄主抗病基因Rxo1介导的水稻对Xanthomonas oryzae pv.oryzicola的抗病反应  

Resistance to Xanthomonas oryzae pv. oryzicola Trigged by Non-host Resistance Gene Rxo1 in Rice

作　　者：王磊[1] 许美容[1] 高晓清[1] 朱苓华[1] 周永力[1] 黎志康[1,2] 

机构地区：[1]中国农业科学院作物科学研究所农作物基因资源与遗传改良国家重大科学工程,北京100081 [2]国际水稻研究所

出　　处：《生物技术通报》2010年第12期100-102,共3页Biotechnology Bulletin

基　　金：国家自然科学基金资助项目(30571200)

摘　　要：通过表型鉴定、反转录PCR和实时定量PCR方法,利用转基因和非转基因水稻植株,研究由Rxol基因介导的,水稻对细菌性条斑病菌的抗性反应。结果观察到3个涉及过敏性反应的基因由Rxol基因诱导表达,并对其进行了分析。这3个基因参与编码病程相关蛋白,在转基因水稻植株中呈上调表达,表明水杨酸信号转导途径在抗性反应中发挥重要的作用。Phenotype,RT-PCR and real time PCR experiments were performed to reveal the resistance reaction of rice to Xanthomonas oryzae pv.oryzicola mediated by Rxo1 using a pair of transgenic and non-transgenic rice lines.Our results indicated that Rxo1 trigged a typical hypersensitive reaction（HR）based on the observation and expression analysis of 3 gene involved in HR.3 genes involved in coding pathogenesis-related proteins also specifically up-regulated in transgenic rice plants,suggesting that salicylic acid（SA）signal transduction pathway played a role in resistance reaction.

关 键 词：非寄主抗性 Rxol 细菌性条斑病菌 

分 类 号：S511[农业科学—作物学]