微卫星标记在穿山甲个体识别中的应用  

Application of Microsatellite Marking to Individual Identification for Pangolins(Manis spp.)

作　　者：高赛飞[1,2] 于冬梅[2] 王莹[1] 彭建军[2] 

机构地区：[1]暨南大学生命科学技术学院,广州510632 [2]华南濒危动物研究所物种鉴定中心,广州510260

出　　处：《生物技术通报》2010年第12期154-157,共4页Biotechnology Bulletin

基　　金：广东省科学院优秀青年科技人才基金项目(200804);广东省野生动物保护和利用公共实验室基金项目(2009001)

摘　　要：在对非法走私和捕杀穿山甲的犯罪案件研究中,所涉及的穿山甲个体数量是量刑的重要依据,然而对于一些穿山甲鳞片、肉块及其深加工产品,从形态学上却无法进行个体识别。微卫星标记已证实是一种用于动物个体识别的可靠的遗传标记。本研究从已发表的34对穿山甲微卫星引物中筛选出6对多态性较高的引物,用于穿山甲鳞片的个体识别。结果表明:6个位点的观测值杂合度(Ho)为0.653-0.234;期望值杂合度(He)为0.926-0.861;MJA03的PIC最高(0.918),MJA13的PIC最低(0.852),平均PIC为0.884。单个座位的个体识别能力在0.826-0.956之间,累积个体识别能力为99.9999%,表明采用的6个位点适用于国内穿山甲走私案中的个体识别。In the criminal cases of poaching and smuggling pangolins（Manis spp.）,the number of individuals involved is critical gist for determining the penalty.Morphological methodologies do not often work because the small cut parts of pangolin do not possess the holistic distinctive characteristics of the individual.Microsatellite DNAs have been proved a reliable marker for the individualization of animals.The DNA was amplified by PCR with 6 polymophic microsatellite as the primer were selected from 34 published microsatellite loci.The results showed that all the 6 microsatellite loci had genetic polymorphisms in pangolins and the polymorphism information content（PIC）in loci MJA03 was the biggest（0.918）,while the MJA13 was the lowest（0.852）.Power of discrimination（DP）value of the 6 STR loci ranged from 0.826 to 0.956.The Cumulate DP（CDP）was 99.9999%.These results indicate that the 6 micro-satellites can be applied to the different individual identification for pangolins.

关 键 词：穿山甲 微卫星标记 个体识别 

分 类 号：S863[农业科学—野生动物驯养] Q789[农业科学—畜牧兽医]