一种PCR酶切克隆目的DNA方法  

A PCR Amplication Method of Combining Endonuclease Digestion

作　　者：王俐[1] 张俊河[1] 董卫华[1] 王芳[1] 王天云[1] 

机构地区：[1]新乡医学院生物化学与分子生物学教研室,新乡453003

出　　处：《生物技术通报》2010年第12期186-188,共3页Biotechnology Bulletin

基　　金：河南省科技厅科技攻关项目(0624410041);河南省教育厅自然科学基金项目(2008A310008)

摘　　要：以PCR扩增克隆survivin启动子为例,根据GenBank报道的序列设计引物,PCR扩增克隆survivin启动子。通过软件在线分析扩增的survivin启动子酶切位点,将扩增与预期的片段大小一致的DNA片段进行酶切,再进行测序。结果表明,采取酶切鉴定PCR产物的方法,可以初步对PCR产物是否正确做出判断。A PCR amplication method of combining endonuclease digestion was reported.When the survivin promoter was amplified,primers were designed according to the survivin promoter sequence and then PCR was carried out.The restriction endonuclease enzymes sites were analyzed by on-line software,and PCR products were digested and then sequenced.Results showed that use the method of enzyme digestion to appraise the product of PCR,it can preliminary to decide the correctness of the product of the PCR.

关 键 词：PCR 引物 酶切 电泳 

分 类 号：Q78[生物学—分子生物学]