基于多重PCR的DNA Marker制备方法  

A Simple Method for Prepareing DNA Marker

作　　者：张俊河[1] 王俐[1] 董卫华[1] 王芳[1] 王天云[1] 

机构地区：[1]新乡医学院生物化学与分子生物学教研室,新乡453003

出　　处：《生物技术通报》2010年第12期189-190,200,共3页Biotechnology Bulletin

基　　金：河南省科技厅科技攻关资助项目(0624410041);河南省教育厅自然科学基金(2008A310008)

摘　　要：报道了一种简便的制备分子量大小为100-1000bp DNA marker的方法,其原理是以一段特异的DNA片段为模板,设计PCR引物,采用多重PCR的方法一次扩增100-1000bp系列条带,酚/氯仿抽提,乙醇沉淀,即可得到条带清晰的DNA marker。A simple method that prepare DNA marker is reported in this paper.A specific DNA fragment was used as PCR template,and multiple PCR was used to amplify 100-1 000 bp series DNA fragments,and then were purified and electrophoresised in 2% agarose gels,the bands of the prepared DNA marker were clear and could be used in the molecular experiments.

关 键 词：DNA MARKER PCR 凝胶电泳 制备 

分 类 号：Q75-3[生物学—分子生物学]