转基因表达OPH提高番茄果实降解有机磷农药能力的研究  

Transgenic Expressing OPH to Enhance the Detoxifying Activity of Tomato Fruit on Organophosphorous Pesticides

作　　者：赵杰宏[1] 赵德刚[1] 韩洁[2] 

机构地区：[1]贵州大学农业生物工程省重点实验室,贵阳550025 [2]贵阳中医学院基础部微生物教研室,贵阳550002

出　　处：《生物技术通报》2010年第12期213-216,225,共5页Biotechnology Bulletin

基　　金：国家科技支撑计划(2007BAD59B00);国际科技合作项目(2007DFA31260);国家转基因新品种培育重大专项(2008ZX08010-003)

摘　　要：拟通过基因工程提高番茄果实降解有机磷农药残留的能力。构建了E8启动子基因驱动有机磷降解基因(OPD)的植物表达载体pSE8OP,经农杆菌介导遗传转化番茄子叶后,进行GUS染色、PCR和Southern blotting分析。证明OPD基因已整合进转基因植株基因组中,为1个拷贝。HPLC比较分析发现,转基因番茄果实能显著提高降解毒死蜱和对硫磷的能力,大大减少了番茄中的农药残留。In order to enhance the degrading ability of tomato fruit on organophosphorous pesticides,we have constructed a plant expression vector of OPD gene which were driven by E8 promoter gene.Then the OPD gene was transformed into tomato cotyledons.It was confirmed preliminarily that the OPD gene has been integrated into the genome of transformed tomato with one copy by GUS staining,PCR and Southern blotting detection.The degrading capability of transgenic tomato on parathion and chlopyrifos was significant improved analyzed by HPLC.It would decrease the pesticide residues in tomato fruit greatly.

关 键 词：遗传转化 有机磷水解酶(OPH)农药残留 毒死蜱 对硫磷 

分 类 号：S641.2[农业科学—蔬菜学]