四株抗荔枝病原菌的芽孢杆菌的分离鉴定  

Isolation and Identification of Four Bacillus Strains with Antagonistic Ability Against Litchi Pathogenic Fungi

作　　者：黄庶识[1,2] 黄曦[1,3] 许兰兰[1,2] 张荣灿[4] 许铭本[4] 黄荣韶[3] 

机构地区：[1]广西科学院生物物理实验室,南宁530007 [2]昆明理工大学生物工程技术研究中心,昆明650224 [3]广西大学农学院,南宁530007 [4]广西科学院海洋环境监测中心,南宁530007

出　　处：《生物技术通报》2010年第12期217-221,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30860010);广西科学基金项目(桂科回0832006);广西科研院所基本科研业务费资助项目(08YJ16WL01)

摘　　要：荔枝果树根际土壤中筛选出4株芽孢杆菌OR-1、OR-2、OR-3、ON-6,都显示出抗荔枝病原菌的活性。采取对该菌株形态特征、培养特征、生理生化特征和遗传特性进行研究的方法,结果表明菌株与枯草芽孢杆菌(Bacillus subtilis)的特征一致;将4菌株的16S rDNA序列在GenBank中进行序列比对,结果亦显示其与Bacillus subtilis的16S rDNA的序列片段的相似性均达99%以上;以相似性为基础构建系统进化树,分析表明菌株与Bacillus subtilis同源关系最近。最终得出结论为菌株OR-1、OR-2、OR-3、ON-6为枯草芽孢杆菌。Four strains of Bacillus OR-1,OR-2,OR-3,ON-6,were isolated from rhizosphere soil of litchi,all of which showed the inhibitory activity of against litchi pathogens fungi.The investigation of their formal,cultural and physiological characteristics indicated that they had the same characteristic with Bacillus subtilis.Comparis on of their 16S rDNA sequence with the data in GenBank,showed the similarity of 99% or more between the four strains and Bacillus subtilis.Phylogenetic tree based on the similarity was established and confirmed that the four strain had the closest relationship with Bacillus subtilis.Finally,the strain OR-1,OR-2,OR-3,ON-6 were identified to be Bacillus subtilis.

关 键 词：枯草芽孢杆菌 荔枝病原菌 分离鉴定 16S rDNA测序 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]