应用随机PCR方法鉴定一株真养产碱杆菌  

Identification of the Genome of an Isolate of Cupriavidus metallidurans Using Random PCR

作　　者：姚四新[1] 赵淑秋[1] 王宪文[1] 王丽荣[1] 赵朴[1] 陈仕钧[1] 张兆沛[1] 王青[1] 刘兴友[1] 刘金华[2] 

机构地区：[1]河南科技学院动物科学学院,新乡453003 [2]中国农业大学动物医学院,北京100094

出　　处：《生物技术通报》2010年第12期222-225,共4页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30972202);河南科技学院院级重点项目

摘　　要：采用随机引物PCR技术从新建细胞培养室空气中获得一段长414bp的片段,通过克隆测序及序列分析,结果表明所测序列与真养产碱杆菌主要参考菌株的同源性分别高达79%-83%,由其推导的氨基酸序列与真养产碱杆菌主要参考菌株的同源性高达86.4%-89.1%,从而确定所分离菌株为真养产碱杆菌。The PCR product of length of 414 bp was derived from air of new cell culture room by using random PCR,and was cloned into pGEM-T vector for sequencing and analysis.Results showed that the identity of the nucleotide sequence of obtained gene between HN09 isolate and other Cupriavidus metallidurans reference strains were from 79%-83%,the identity of the deduced amino acid sequence of obtained gene between HN09 isolate and other Cupriavidus metallidurans reference strains were from 86.4%-89.1%.The present study indicated that HN09 isolate may be a member of Cupriavidus metallidurans.

关 键 词：植物真养产碱杆菌 随机引物 随机PCR 

分 类 号：Q93-33[生物学—微生物学]