铜绿假单胞菌的基因芯片检测研究  

Gene chips micro array in Pseudomonas aeruginosa detection

作　　者：瞿良[1] 王惠萱[1] 谭德勇[2] 程罕松[3] 

机构地区：[1]解放军昆明总医院检验科,云南昆明650032 [2]云南大学生物化学与分子生物学生物技术系,云南昆明650031 [3]中山大学达安基因股份有限公司,广东广州510070

出　　处：《国际检验医学杂志》2010年第11期1201-1202,1206,共3页International Journal of Laboratory Medicine

基　　金："863"国家高技术研究发展计划资助项目(2006AA020902)

摘　　要：目的用基因芯片对临床标本铜绿假单胞菌进行快速检测。方法结合临床检验经验,抽取98例临床标本,其中78例常规细菌培养鉴定疑似铜绿假单胞菌感染,20例非铜绿假单胞菌感染,铜绿假单胞菌质控菌株(ATCC27853),大肠埃希菌(ATCC25922),用研究制备出铜绿假单胞菌筛查的基因芯片进行测定,同时进行比对分析。结果铜绿假单胞菌检出符合率为100%,基因芯片检测的标本需要量少,检验时间短,敏感性、特异性、准确性高。结论针对细菌培养的临床标本特点,采用高效的基因芯片检测方法,具有现实的临床意义。Objective To discuss the feasibility of rapid detection of Pseudomonas aeruginosa in clinical specimens that by gene chip. Methods In clinical examination experience,98 cases of clinical specimens were collected,including 78 suspected cases of Pseudomonas aeruginosa infections with conventional bacterial culture and identification,20 cases of non-Pseudomonas aeruginosa, quality control strain of Pseudomonas aeruginosa（ATCC27853） and Escherichia coli （ATCC25922）. Gene chip screening in Pseudomonas aeruginosa was measured at the same time to compare the analysis. Results The positive rate in detection of Pseudomonas aeruginosa was 100 % ,gene chips required less testing of specimens, and testing time is short, with high sensitivity, specificity and accuracy. Conclusion For clinical specimens,using highly efficient gene chip detection method to detect Pseudomonas aeruginosa has practical clinical significance.

关 键 词：假单胞菌 铜绿 寡核苷酸序列分析 实验室技术和方法 

分 类 号：R440[医药卫生—诊断学]