确认试验在ELISA法筛查梅毒抗体阳性中的应用价值  

Evaluate on application of the confirmatory test for positive samples with treponema pallidum antibody by ELISA screening

作　　者：刘孙琴[1] 韩晓峰[1] 门诚虹[1] 关崇霞[1] 杨明建[1] 刘蔚[1] 

机构地区：[1]武汉科技大学附属天佑医院检验科,湖北武汉430064

出　　处：《中国当代医药》2010年第33期77-78,共2页China Modern Medicine

摘　　要：目的:探讨确认试验在酶联免疫吸附试验(ELISA)法筛查梅毒抗体阳性中的应用价值,提高梅毒检测的准确性。方法:先用ELISA法对7112例患者的血清进行检测,阳性标本再用梅毒螺旋体明胶颗粒凝聚试验(TPPA)和免疫印迹法(WB)进行确认试验。结果:ELISA法检出110例阳性,TPPA法确认107例阳性,假阳性率为2.72%(3/110);WB法确认106例阳性,假阳性率为3.64%(4/110);WB法与TPPA法检出假阳性率的差异无统计学意义(χ2=0.56,P>0.05)。结论:为提高梅毒检测的准确性,对ELISA法筛查的阳性标本,结合临床表现和病史,用TPPA法进行确认试验,对有疑问的结果用WB法进一步确认。Objective：To evaluate on application of the confirmatory test for positive samples with treponema pallidum antibody by ELISA screening,to improve the accuracy of detection of treponema pallidum.Methods：Detected treponema pallidum antibody in 7 112 patients by ELISA,then positive samples were confirmed by TPPA and WB methods.Results：The 110 cases positive samples were detected by ELISA,107 cases were confirmed positive by TPPA method,the falsepositive rate was 2.73%（3/110）;106 cases were confirmed positive by WB method,the false-positive rate was 3.64%（4/110）.The difference of detection false-positive rate between WB and TPPA was not statistically significant（χ^2=0.56,P〉0.05）.Conclusion：To improve the accuracy of detection of treponema pallidum,the positive samples with treponema pallidum are screened by ELISA,to combine with the clinical diagnosis and disease history,to confirm by TPPA,question results is confirmed further by WB method.

关 键 词：梅毒螺旋体 酶联免疫吸附试验 梅毒螺旋体明胶颗粒凝聚试验 免疫印迹法 确认试验 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]