聚磷菌富集实验及其内源特征探究  

Enriched experiment and endogenous characteristics of polyphosphate-accumulating organisms(PAOs)

作　　者：王啟林[1] 郝晓地[1] 曹亚莉[1] 

机构地区：[1]北京建筑工程学院,城市雨水系统与水环境省部共建教育部重点实验室/可持续环境生物技术研发中心,北京100044

出　　处：《环境科学学报》2010年第12期2405-2413,共9页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金项目(No.50678017);北京市属高等学校人才强教-深化计划高层次人才项目(No.PHR20100508)~~

摘　　要：通过磷酸盐释放速率(PRR)测定、荧光原位杂交技术(FISH)及LIVE/DEAD细胞染色技术,分别研究了生物营养物去除(BNR)系统与富集聚磷菌(PAOs)序批式反应器(SBR)系统中PAOs在好氧环境下的衰减特征.结果表明,当富集聚磷菌SBR系统进料中碳源(三水合乙酸钠和丙酸)是以36d为一个循环周期方式投加时,即三水合乙酸钠24d和丙酸12d,系统中PAOs富集比例可达91%.测定与计算结果表明,生物营养物去除(BNR)系统与富集聚磷菌SBR系统中PAOs衰减速率分别为0.113d-1和0.181d-1,死亡速率分别为0.048d-1和0.036d-1.这说明由细胞死亡引起的PAOs数量衰减在两个系统中分别占细胞总衰减的42%(BNR)和20%(SBR),而由细胞活性降低引起的活性衰减分别占细胞总衰减的58%(BNR)和80%(SBR).由此可见,PAOs数量衰减在其细胞总衰减中只占较小一部分,而绝大部分衰减是由活性衰减所引起.By means of measuring maximal anaerobic phosphate release rates（PRR）,analyzing 16S rRNA with fluorescence in-situ hybridization（FISH） and observing membrane integrity by live/dead staining,the aerobic decay characteristics of polyphosphate-accumulating organisms（PAOs） in a biological nutrient removal（BNR） system and an enriched PAOs sequencing batch reactor（SBR） system were investigated.It was experimentally determined that a highly enriched culture of PAOs（91%） was obtained in the enriched PAO-SBR system by alternating the carbon source in the feed between sodium acetate trihydrate and propionate over a 36 d switching cycle,i.e.,sodium acetate trihydrate（24 d） and propionate（12 d）.The experimental results and calculations revealed that the decay rates of PAOs in the BNR and enriched PAO-SBR systems were 0.113 d-1 and 0.181 d-1,respectively,and the death rates were 0.048 d-1 and 0.036 d-1.In addition,the results also demonstrated that cell death contributed 42% and 20%,respectively,to the cell decay in the BNR and enriched PAO-SBR systems.In other words,the activity decay accounted for 58% and 80% of the total cell decay.For this reason,cell death was not a major factor causing the cell decay of PAOs,but activity decay was mostly responsible for this process.

关 键 词：内源过程 细胞衰减 细胞死亡 活性衰减 磷酸盐释放速率(PRR) 荧光原位杂交(FISH) LIVE/DEAD染色 

分 类 号：X703.1[环境科学与工程—环境工程]