基于mtDNA 16S rRNA序列的脉红螺(Rapana venosa)与红螺(R.bezoar)的分类学研究  

TAXONOMIC RELATIONSHIP BETWEEN RAPANA VENOSA AND RAPANA BEZOAR BASED ON 16S rRNA GENE SEQUENCES

作　　者：杨建敏[1,2] 郑小东[2] 李琪[2] 王如才[2] 王卫军[1] 孙国华[1] 张宇[1] 

机构地区：[1]山东省海洋水产研究所,烟台264006 [2]中国海洋大学水产学院,青岛266003

出　　处：《海洋与湖沼》2010年第5期748-755,共8页Oceanologia Et Limnologia Sinica

基　　金：国家"863"计划"中国近海贝类资源的采集;保存与信息化技术";2007AA09Z433号

摘　　要：采用线粒体DNA16SrRNA基因序列测定方法,对中国沿海3目8科的14种腹足类的系统演化关系进行了比较分析。结果显示,共测定了102个个体,获得线粒体DNA16SrRNA基因片段507bp的同源碱基序列;用最大简约法检测到保守位点87个,可变位点409个,简约信息位点254个;计算了碱基替换/颠换率的距离(R)和基于碱基替换+颠换率的距离(D),红螺属内的脉红螺和红螺R值为0.9367,大于0.5,属于种间标志值;采用Kimura2-parametermodel构建了邻接法(NJ)、最小进化法(ME)和最大似然法(MP)等3种系统树,脉红螺和红螺各自均为单系群(支持率=92—100),同时表明它们在检测的14种腹足类中属于相当进化种类。分析结果从线粒体基因序列的层次支持脉红螺与红螺属于不同种类的现代分类方法。14 marine gastropods, belonging to 3 orders and 8 families, were phylogenically analyzed based on partial sequences of the mitochondrial 16S rRNA gene. As a result, a homologous nucleotide sequence with 507 base pair was obtained from 102 individuals; 87 conserved sites, 409 variable sites and 254 parsim-info sites were discovered by the method of Maximum Parsimony; D （transition＋transversion）-distance and R （transition/transversion）-distance were calcu- lated, and the value R was 0.9367 （0.5） between Rapana venosa and R. bezoar, which showed different species; Neighbor-Joining, Minimum Evolution and Maximum Parsimony trees were constructed based on the Kimura 2-parameter model, which supported that R. venosa and R. bezoar belonged to homogroup （support values： 92—100）; and R. venosa and R. bezoar were also much higher than the other 12 gastropods in evolutionary level. In a word, R. venosa and R. bezoar belonged to different species based on mtDNA 16S rRNA gene sequences data, which was in of accord with modern tax- onomy.

关 键 词：脉红螺 红螺 16S RRNA基因 系统发生 

分 类 号：Q346[生物学—遗传学]