中国对虾(Fenneropenaeus Chinensis)自然感染与人工感染WSSV抗病力的比较  

COMPARISON OF WSSV-RESISTANCE IN ARTIFICIAL AND NATURAL INFECTION EXPERIMENTS IN FENNEROPENAEUS CHINENSIS

作　　者：张天时[1,2] 李素红[2,3] 孔杰[2] 王清印[2] 孟宪红[2] 罗坤[2] 

机构地区：[1]中国海洋大学生命科学与技术学部,青岛266003 [2]中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室,青岛266071 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《海洋与湖沼》2010年第5期763-768,共6页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金项目;30871919号;国家863计划项目;2006AA10A406号

摘　　要：取30个通过人工授精和自然交尾建立的中国对虾家系,分别进行人工感染和自然感染实验。实验过程中,两实验组样本总体存活率在35%左右时统计各家系的存活率,以分析其抗白斑综合征病毒情况。用四格表的卡方检验分析中国对虾各家系存活率,结果发现23个家系的存活率在两种感染方式实验间差异不显著(P>0.05),两种感染方式实验间家系存活率一致性为77%。用独立样本T检验对两种感染方式实验中4个杂交组合的存活率进行比较,结果发现4个杂交组合的存活率在两种感染方式实验间差异不显著(P>0.05),说明人工感染和自然感染实验对中国对虾抗病力的反映情况基本是一致的。另外,对两个实验中各家系的抗病力进行分析,综合结果表明有5个家系对WSSV可能有较强的抗病力。Thirty families of Fenneropenaeus chinensis produced by natural mating or artificial insemination were in- fected with WSSV by artificial or natural infection. In order to compare the WSSV-resistance among families, survival rates of each family were counted when survival rates of total samples approximately reached 35 percent during the course of the two experiments. Chi-square test for fourfold table was applied to analyze the survival rate of each family. Survival rates of 23 families were consistent in two infection experiments, with family survival rate of 77% consistency. The result of independent-sample T test for survival rates of each cross combination showed that there were not significant differ- ences between the two infection experiments (P0.05), which indicated that the WSSV-resistance of each family were al- most consistent between the artificial infection and the natural infection experiments. In addition, WSSV-resistance was analyzed in all the families in two experiments, and the result indicated that 5 families probably were better than those of the others in WSSV-resistance.

关 键 词：人工感染 自然感染 存活率 抗白斑综合征病毒 

分 类 号：S945.46[农业科学—水产养殖]